GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Methylthiotransferase, die die Umwandlung von N6-(Dimethylallyl)adenosin (i6A) in 2-Methylthio-N6-(Dimethylallyl)adenosin (ms2i6A) an Position 37 (neben dem 3'-Ende des Anticodons) von vier für mitochondriale DNA kodierten tRNAs (Ser(UCN), Phe,
Wirtsspezies
Kaninchen
Spezifität
CDK5RAP1
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDK5RAP1.
Positive Kontrolle
WB: Mouse lung lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
Carrier Free, ExactAb™, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Immunogen affinity purified
Konzentration
1.0 mg/mL
Lagerung und Versand
Form
Flüssigkeit
Storage
Bei -20°C lagern, wiederholtes Einfrieren und Auftauen vermeiden
Verschickt in
Eistruhe + Eispads
Stabilität und Lagerung
Kurzfristig bei 4°C lagern (1-2 Wochen). Langfristig bei -20°C lagern (12 Monate). Es wird empfohlen, nach Erhalt zu aliquotieren. Einfrier-/Auftauzyklus vermeiden.
Bilder
Recombinant CDK5RAP1 Antibody (Ab095771) - Western Blot All lanes: Recombinant CDK5RAP1 Antibody (Ab095771) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 68 kDa Observed band size: 61 kDa Exposure time: 27.0 s
Recombinant CDK5RAP1 Antibody (Ab095771) - IHC Immunohistochemical analysis of CDK5RAP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant CDK5RAP1 Antibody (Ab095771) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CDK5RAP1 Antibody (Ab095771) - IF/ICC IF analysis of CDK5RAP1 Antibody (Ab095771) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CDK5RAP1 Antibody (Ab095771) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
IHC
1/50-1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Wir verwenden Cookies, um die ordnungsgemäße Funktion der Website sicherzustellen und, soweit zulässig, Ihr Nutzererlebnis zu verbessern. Sie können Ihre Einstellungen jederzeit unter „Einstellungen“ verwalten. Weitere Informationen finden Sie in unserer Cookie-Richtlinie.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F1013136