Clostridium perfringens Counting Agar - BioReagent

Cat. No.: C1528674

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Übersicht

For enumeration of anaerobic sulfite Clostridium (Japan)

Specifications

Spezifikationen & Reinheit

BioReagent

Storage

Room temperature

Verschickt in

Normal

Note

BioReagent

Product Line

Biologische Produkte

Application Protocols

No manufacturer‑validated protocols are provided in the Product Data. The following are general, literature‑based outlines; adjust to your governing method and this item’s label/CoA.

• Pour plate (literature):

1. Prepare and sterilize medium per label. Cool to ~45–50 °C.
2. Pipette measured sample/decimal dilutions into sterile Petri dishes.
3. Add molten medium, mix gently, allow to solidify; overlay if required to ensure anaerobiosis.
4. Incubate anaerobically at the method‑specified temperature/time. Count characteristic colonies and calculate CFU.

• Membrane filtration (literature):

1. Filter defined sample volume through 0.45 µm membrane.
2. Place membrane on prepared plates; incubate anaerobically.
3. Enumerate characteristic colonies; transfer for confirmation as required.

• Controls and confirmation (literature): Include positive control strain(s) of C. perfringens and appropriate negative controls. Perform confirmatory biochemical/molecular assays per standard.

Note: Exact supplement requirements, temperatures, incubation times, and colony criteria are method‑specific and Not specified for this item; refer to CoA/Spec Sheet and your chosen standard (e.g., ISO/BAM/APHA).

Biological Roles

This product is not a biomolecule but a nutrient/selective matrix to support cultivation and enumeration of Clostridium perfringens.

• Functional role (literature): Provides carbon/nitrogen sources, minerals, and selective/differential agents tailored to favor recovery of C. perfringens under anaerobic conditions while suppressing non‑targets. Agar supplies a solid phase for colony isolation and counting. • Selective/differential mechanisms (literature): Commonly exploit sulfite reduction and iron salt precipitation (black colonies), and may include lecithinase detection with egg yolk, reflecting phospholipase C activity characteristic of C. perfringens. Exact composition of this item is Not specified; consult CoA/label before assuming mechanism. • Microbial physiology context (literature):

• C. perfringens is an obligate anaerobe; redox potential and oxygen scavenging capacity of the system influence recoveries.
• Growth temperature window is typically mesophilic; exact setpoints depend on the chosen standard method (often 35–46 °C). • Validation: Enumeration on selective agar is often paired with confirmatory biochemical or molecular tests to ensure specificity, as related clostridia can exhibit similar colony morphologies.

Buffer Applications

Not typically applicable. This product is a dehydrated culture medium formulated for microbial growth on solid agar, not a chemical buffering system. For pH control during preparation, follow the method‑specific guidance and any pH range provided on the label/CoA. If a buffer is required for confirmatory tests, select according to the specific assay protocol.

Green Alternatives

While “green chemistry” solvent substitution is not directly relevant to culture media, laboratories can reduce environmental impact through media handling and method choices.

• Dehydrated vs. ready‑to‑use plates:

• Dehydrated media (this item): Lower packaging waste and shipping mass; longer shelf life at ambient storage. Requires energy for autoclaving and plate pouring.
• Ready‑to‑use plates: Eliminate in‑house sterilization energy/water but increase transport volume and cold‑chain needs. • Anaerobiosis approaches (literature):
• Use reusable anaerobic jars with catalyst sachets rather than single‑use pouches when feasible.
• Validate microaerobic/anaerobic workstations to minimize disposable sachet consumption for high‑throughput labs. • Energy/water efficiency:
• Batch sterilize and pour to maximize autoclave loads and reduce cycle counts.
• Employ validated shorter sterilization cycles where permitted by method and load type to save energy. • Waste minimization:
• Right‑size batch volumes to reduce expired plates.
• Autoclave used/inoculated media efficiently; segregate non‑biohazard packaging for recycling where allowed. • Chemical substitutions: If your method allows, antibiotic‑reduced formulations or selective supplements at lower load may decrease hazard profile; always adhere to the governing standard method and performance criteria.

Pharmaceutical Uses

No therapeutic or clinical use is implied. In pharmaceutical and biomanufacturing laboratories, Clostridium perfringens counting media may be used in quality control or environmental monitoring programs to assess or study anaerobic contamination risks (literature). Any use in GMP settings requires user qualification/validation.

• Potential roles (literature):

• Method development or suitability testing for anaerobic bioburden assessments.
• Training and proficiency testing for anaerobic culturing techniques. • Compliance considerations:
• This item is supplied as BioReagent grade; it is not claimed to meet pharmacopeial monographs. Users must establish fitness for intended use and maintain appropriate documentation. • Packaging/traceability: Refer to CoA/Spec Sheet for lot‑specific data (e.g., recommended preparation concentration, pH of prepared medium, performance testing, and expiry).

Physical Properties

Item-specific physical constants for this complex medium are not defined as single values. Where needed, consult the product label and CoA.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet. • Solubility: Readily dispersible in water during medium preparation (general property of dehydrated agar media; literature). Final gel forms upon cooling after sterilization. • pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet. Typical target pH for C. perfringens counting media after sterilization is ~7.0–7.6 at 25 °C (literature; varies by standard method). • Bulk density / moisture: Not specified for this item; refer to CoA/Spec Sheet. • Melting/boiling points, refractive index, logP: Not applicable to complex dehydrated mixtures; agar gels undergo thermoreversible sol–gel transitions (gelation typically ~35–45 °C; melting ~85–95 °C; literature for agar). • Preparation concentration: Not specified for this item; refer to label/CoA. Many counting media are prepared in the range of ~40–60 g/L (literature, method‑dependent).

Quality and Grades

• Supplied grade: BioReagent (per Product Data). This indicates suitability for general laboratory and microbiology workflows, with controlled raw materials and manufacturing suitable for research applications. It is not intended for clinical diagnostic use unless otherwise validated by the user. • What BioReagent means in practice:

• Consistent performance in culture workflows (e.g., colony recovery, selectivity) when prepared as directed.
• Manufactured for laboratory use with attention to lot‑to‑lot reproducibility.
• Not certified to pharmacopeial monographs unless explicitly stated. • Item-specific specifications (e.g., pH of prepared medium, productivity/selectivity, inhibitory substances, moisture content): Not specified for this item; refer to CoA/Spec Sheet. • Stabilizers/indicators: Not specified for this item; refer to CoA/Spec Sheet. Some C. perfringens counting media in the literature utilize sulfite–iron systems and may incorporate egg yolk emulsion or antibiotics; do not assume presence without checking the CoA/label. • User QC suggestions (literature guidance):
• Performance testing with control strains (e.g., Clostridium perfringens ATCC strains) under anaerobic conditions.
• pH verification after sterilization.
• Visual inspection for homogeneity, absence of caking, and correct gel strength after preparation. • Shipping/storage per Product Data: Shipped at ambient; store at room temperature in a tightly closed container, dry environment.

Reaction and Applications

Chemical reaction content is not applicable. Instead, practical microbiology applications are summarized below.

• Intended use (literature): Enumeration of Clostridium perfringens by pour plate or membrane filtration methods under anaerobic incubation. Used in food, water, and environmental testing per various standards (e.g., ISO 7937, BAM, APHA methods; users should consult the specific method they follow). • Principle (literature): Many C. perfringens counting agars are selective/differential via sulfite reduction; colonies appear dark/black due to iron sulfide precipitation. Some formulations include lecithinase detection with egg yolk, producing opaque halos; antibiotics may suppress competing flora. Do not infer specific formulation for this item—check the label/CoA. • Workflow notes (literature):

• Prepare medium per label; pour plates or overlay as required by the method.
• Inoculate samples or membranes, solidify, and incubate anaerobically (commonly 35–46 °C depending on method and regulatory standard) for 18–48 h.
• Confirm presumptive colonies by additional biochemical tests (e.g., lactose fermentation, motility, Nagler test, toxin gene PCR) as required by your method. • Performance considerations: Strict anaerobiosis, correct incubation temperature, and prompt processing of samples are critical to suppress outgrowth of non‑targets and maintain selectivity. • Limitations: Stress/injury to cells (e.g., chlorination, refrigeration) may reduce recoveries; consider resuscitation steps where methods prescribe.

Reaction Conditions

Classical chemical reaction parameters are not applicable. The following summarizes general, literature‑based preparation and incubation conditions for C. perfringens counting workflows. Always defer to the product label/CoA and your governing method.

• Medium preparation (literature, general):

• Suspend the specified mass of dehydrated medium in purified water (concentration Not specified for this item; refer to label/CoA; many media are ~40–60 g/L).
• Heat with agitation to dissolve; adjust pH if directed by the method.
• Sterilize by autoclaving (typical: 121 °C for 15 min; literature). Cool to 45–50 °C before pouring plates. If supplements (e.g., egg yolk, antibiotics) are required by the method, aseptically add at the recommended temperature. • Incubation (literature, method‑dependent):
• Inoculate and incubate under strict anaerobic conditions using jars, pouches, or anaerobic chambers.
• Temperature/time vary by standard (commonly 35–46 °C for 18–48 h). Overlay techniques may be specified to maintain anaerobiosis in plates. • Quality checks: Verify sterility of uninoculated controls; check plate surface quality and absence of excessive condensation. • Expected outcomes: Target colonies with morphology/color characteristic of the selected method; confirm presumptives by secondary tests per protocol.

Safety and Handling

• GHS/Classification: Not specified for this item; refer to SDS for authoritative hazard information. • Signal word / H‑statements / pictograms: Not specified for this item; refer to SDS. • General hazards (literature/good practice): Dehydrated culture media may generate nuisance dust; avoid inhalation. May cause mild eye/skin irritation. Not for human or animal consumption. For research use only. • Microbiological risk: The product itself is non‑pathogenic medium. However, once inoculated with environmental/food samples, plates become biohazardous. Handle incubated materials in a biological safety cabinet as appropriate for potential anaerobic pathogens (e.g., BSL‑2 practices for C. perfringens work; institutional guidance). • Incompatibilities: Avoid moisture uptake before use; clumping reduces performance. Store away from strong oxidizers and acids/bases that could alter medium chemistry (general precaution for complex media). • PPE: Laboratory coat, safety glasses, disposable gloves. Use dust mask/respirator if generating airborne particulates during bulk handling per SDS. • First aid (overview; defer to SDS): Rinse eyes/skin with water for several minutes if contacted. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical advice if symptoms occur. • Spills/Waste: Sweep/HEPA vacuum dry powder without dust generation. Autoclave or disinfect used/inoculated media before disposal per local biosafety regulations. • Fire: Most components are organic; treat as combustible dust. Use water spray, CO2, foam, or dry chemical per SDS.

Solvent Selection

This product is a dehydrated microbiological medium intended to be dissolved in water; conventional solvent selection for organic synthesis does not apply.

• Solvent for preparation: Purified or deionized water (literature). Avoid high‑ionic‑strength waters or residual disinfectants (chlorine/chloramine) that can inhibit growth. • Miscibility: Components are formulated to fully disperse/hydrate in hot water during medium preparation; agar provides thermoreversible gelling upon cooling (literature). • Practical guidance:

• Use freshly distilled/deionized water with conductivity within your lab’s microbiology standards.
• Heat with agitation to achieve complete dissolution; avoid scorching.
• Adjust pH, if specified by the method, prior to autoclaving. • Comparison to alternatives (literature): Ready‑to‑use plates offer convenience and reduce preparation variability but have shorter shelf life and higher cost; dehydrated media offer flexibility, lower cost per plate, and long dry‑storage stability.

Storage and Reconstitution

• Storage conditions (as supplied): Room temperature; shipped under normal conditions (per Product Data). Keep container tightly closed in a cool, dry place. Protect from moisture to prevent caking and loss of performance. • Shelf life: Not specified for this item; refer to CoA/Spec Sheet. • Reconstitution/preparation: Item‑specific mass per liter is Not specified for this item; refer to label/CoA. General literature guidance for dehydrated counting agars:

• Disperse the indicated mass in purified water with agitation.
• Heat to dissolve completely; avoid localized overheating.
• Adjust pH if directed by method; bring to final volume.
• Sterilize by autoclaving (typical literature condition: 121 °C for 15 min). Cool to ~45–50 °C for pouring.
• If required by the method, aseptically add selective/differential supplements at the recommended temperature. • Prepared media storage (literature): Store poured plates at 2–8 °C in sealed sleeves to minimize dehydration and oxidation; use within the method‑recommended window (often days to weeks depending on formulation). Warm to room temperature and dry plate surfaces before use. • Freeze–thaw: Do not freeze dehydrated powder. Avoid repeated thermal cycling of prepared media; prepare fresh as needed. • Research use: For research use only (per Product Data). Always consult the SDS and CoA for lot‑specific handling and performance information.

Structure and Identity

• Item: Clostridium perfringens Counting Agar (dehydrated culture medium) • Nature of material: complex, multi‑component microbiological medium; not a single defined chemical entity. • Molecular formula: Not applicable to complex media; Not specified for this item; refer to CoA/Spec Sheet. • Molecular weight: Not applicable to complex media; Not specified for this item; refer to CoA/Spec Sheet. • SMILES / InChIKey / CAS / CID: Not applicable for complex mixtures; Not specified for this item; refer to CoA/Spec Sheet. • Structural features (descriptive): As a dehydrated agar medium, the product typically contains a gelling polysaccharide (agar) and nutrient/indicator components formulated to support and enumerate Clostridium perfringens under selective, anaerobic conditions (literature). No discrete 2D molecular structure represents the product. • Category: Microbiological culture medium for laboratory use (enumeration of C. perfringens in samples such as foods, water, and environmental materials; literature).

Synthetic Utility

Not applicable. This product is a complex microbiological medium and is not used as a reagent or intermediate in chemical synthesis. For practical laboratory relevance, see Reaction & Applications (microbiological) and Storage & Reconstitution for preparation guidance.

Target Specificity

The Target Specificity section for antibodies/biological binders is not applicable. This product is a culture medium without antigen/epitope specificity. For intended microbiological selectivity, see Reaction & Applications and Biological Roles (literature context).

Häufig gestellte Fragen

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.