GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Native?Native grade — protein/biomolecule in its natural (non-recombinant, non-denatured) form. Use when native structure and activity are required.≥90%(SDS-PAGE)Protein concentration: 0.95-1.2 mg/ml
Accession #
P01031
Bioactivity
Titer >100,000 C5H50 U/mg; >60% of C5 activity in NHS on a mg/mg basis.
Bioactive, BioReagent, Native, ≥90%(SDS-PAGE), Protein concentration: 0.95-1.2 mg/ml Bioactive,BioReagent,Native for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Complement C5 is part of the terminal sequence in the complement pathway. In particular, it is cleaved into C5a and C5b which are responsible for chemotaxis, inflammation, and initiating the formation of the membrane attack complex (MAC). Drugs that inhibit complement C5 have been used in Paroxysmal nocturnal hemoglobinuria (PNH) patients to lessen thrombotic complications by lessening intravascular hemolysis.
Complement component C5 is processed by convertase enzymes in a cascade modulated in a unique way. The multi-subunit catalytic complex initially shows little activity against C5, but instead cleaves C3. A C3 cleavage product C3b covalently attaches to the complex and shifts its specificity to C5 by a factor of 1000.
Purity: >=95% by SDS-PAGE.
Reacts to C5 antisera on Western Blot
Not tested for biological activity.
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Bioactive, BioReagent, Native, ≥90%(SDS-PAGE), Protein concentration: 0.95-1.2 mg/ml
Biochemical and Physiological Mechanisms
Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated
Bioactivity
Titer >100, 000 C5H50 U/mg; >60% of C5 activity in NHS on a mg/mg basis.
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Store at -80℃ long term (3 years). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Unit definition
One C5H50 unit measured by classical pathway activation is defined as the amount of C5 required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C5-Dpl serum for 30 minutes at 37°C in a total reaction volume of
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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