CSRP1 Antibody - Primärer Antikörper, specific to CSRP1, Rabbit IgG

Cat. No.: Ab097983

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Huhn, Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human CSRP1 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.  

Specifications

Product Name

CSRP1 Antibody - Primärer Antikörper, specific to CSRP1, Rabbit IgG

Synonyme

CRP antibody | CRP1 antibody | CSRP 1 antibody | CSRP antibody | CSRP1 antibody | CSRP1_HUMAN antibody | CYRP antibody | Cysteine and glycine rich protein 1 antibody | CRP 1 antibody | Cysteine and glycine-rich protein 1 antibody | Cysteine rich protein 1

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemische und physiologische Mechanismen

Could play a role in neuronal development.

Wirtsspezies

Kaninchen

Spezifität

CSRP1

Positive Kontrolle

WB: Mouse lung, HeLa, A549, NIH/3T3 and C6 lysates. IHC: Human brain tissue. IF/ICC: BV2 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: CSRP1 Antibody (Ab097983)

CSRP1 Antibody (Ab097983) - Western Blot
All lanes: CSRP1 Antibody (Ab097983) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa
Exposure time: 15.0 s

Western Blot: CSRP1 Antibody (Ab097983)

CSRP1 Antibody (Ab097983) - Western Blot
All lanes: CSRP1 Antibody (Ab097983) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 24 kDa
Exposure time: 8.0 s

IHC: CSRP1 Antibody (Ab097983)

CSRP1 Antibody (Ab097983) - IHC
Immunohistochemical analysis of CSRP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with CSRP1 Antibody (Ab097983) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: CSRP1 Antibody (Ab097983)

CSRP1 Antibody (Ab097983) - IF/ICC
Immunofluorescent analysis of CSRP1 staining in BV2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CSRP1 Antibody (Ab097983) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/50 - 1/200;

Zugehörige Ziele (menschlich)

CSRP1 Tbio Cysteine and glycine-rich protein 1 (0 Activities)

Discover Target: CSRP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen