Cyanine 3 Tyramide - ≥95%(HPLC) , CAS No.174961-75-2

CAS: 174961-75-2 Cat. No.: C287079 Molecular Weight: 749.94 PubChem CID: 146018932

GRADE & PURITY ≥95%(HPLC)

Synonyms

2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt | Tyramide-Cy3

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids

Application：

Tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH

Spectral characteristics:

Emission Color                            Orange

λabs                                        550 nm

λem                                        563 nm

Extinction Coefficient (ε)           150000 M-1cm-1

Quantum Yield (φ)                0.31

Closest Laser line                532 nm

Specifications

Synonyms

2-[3-[(1-Ethyl-1, 3-dihydro-3, 3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3, 3-dimethyl-5-sulfo-3H-indolium inner salt | Tyramide-Cy3

Specifications & Purity

≥95%(HPLC)

Biochemical and Physiological Mechanisms

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥95%(HPLC)

Names and Identifiers

• Pubchem Sid: 504773614
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504773614
• Canonical Smiles: CCN1C2=C(C=C(C=C2)S(=O)(=O)O)C(C1=CC=CC3=[N+](C4=C(C3(C)C)C=C(C=C4)S(=O)(=O)[O-])CCCCCC(=O)NCCC5=CC=C(C=C5)O)(C)C
• IUPAC Name: 2-[(E,3E)-3-(1-ethyl-3,3-dimethyl-5-sulfoindol-2-ylidene)prop-1-enyl]-1-[6-[2-(4-hydroxyphenyl)ethylamino]-6-oxohexyl]-3,3-dimethylindol-1-ium-5-sulfonate
• InChIKey: JPXIOSIRIWBDIC-UHFFFAOYSA-N
• INCHI: 1S/C39H47N3O8S2/c1-6-41-33-20-18-29(51(45,46)47)25-31(33)38(2,3)35(41)11-10-12-36-39(4,5)32-26-30(52(48,49)50)19-21-34(32)42(36)24-9-7-8-13-37(44)40-23-22-27-14-16-28(43)17-15-27/h10-12,14-21,25-26H,6-9,13,22-24H2,1-5H3,(H3-,40,43,44,45,46,47,48,49,50)
• PubChem CID: 146018932
• Molecular Weight: 749.94

Chemical and Physical Properties

• Solubility: Solvent:DMSO, Max Conc. mg/mL: 12, Max Conc. mM: 16