Cysteine (Cys) Content Assay Kit (PTA, Micro Method)

Cat. No.: C1515952

GRADE & PURITY BioReagent

Übersicht

There are three sulfur-containing amino acids in proteins: methionine, cystine, and cysteine (Cys). Among them, Cys is the only sulfur-containing amino acid that contains a sulfhydryl (-SH) group. It is derived from methionine and can be interconverted with cystine. Cys participates in the formation of protein disulfide bonds, often constitutes part of the active site of proteins, and can provide sulfhydryl groups for other physiological and biochemical reactions. Furthermore, Cys accumulates abundantly on the surface of the skin and mucous membranes. It maintains the activity of crucial sulfhydryl enzymes involved in keratin production, replenishes sulfhydryl groups to support normal skin metabolism, and regulates the production of underlying melanin by pigment cells in the deepest layer of the epidermis. These functions contribute to its roles in skin whitening, detoxification, and improving inflammatory and allergic skin conditions. Assay Principle: Cys reduces phosphotungstic acid to produce tungsten blue, which exhibits an absorption peak at 600 nm. The Cys content is calculated based on the absorbance at 600 nm.

Specifications

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Anwendung

Cell Metabolism

Probentyp

Bakterien, Pflanzengewebe, Plasma, Serum, Tierische Gewebe

Methode des Nachweises

Colorimetric Method

Detection instrument

Microplate reader,Visible Spectrophotometer

Detection wavelength

600nm

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ for 6 months. Store in the dark.

Häufig gestellte Fragen

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.