BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Vitamin C, also known as L-Ascorbic Acid (AsA) , is an essential nutrient for higher primates and a few other organisms. In living organisms, Vitamin C acts as an antioxidant; it is an acidic hexose derivative and a lactone of an enolic sugar acid, protecting the body from damage by free radicals. It also functions as a coenzyme. Its widespread dietary sources are various fresh fruits and vegetables. Vitamin C has two isomers, the L-form and the D-form, but only the L-form possesses physiological function. Both the reduced and oxidized forms exhibit physiological activity. The detection principle of this kit is as follows: A reducing agent is used to convert dehydroascorbic acid to reduced ascorbic acid. Under acidic conditions, the reduced ascorbic acid reduces ferric ions (Fe³⁺) to ferrous ions (Fe²⁺), which then form a stable red chelate complex with phenanthroline. The absorbance is measured at 534 nm using a spectrophotometer. Within a specific concentration range (sample concentration controlled between 1-40 μg/ml), the absorbance shows a linear relationship with the ascorbic acid content, allowing for the determination of ascorbic acid concentration. This kit is primarily used for detecting Vitamin C (ascorbic acid) in plant tissues. It measures the total ascorbic acid content; by subtracting the original reduced ascorbic acid content present in the sample, the dehydroascorbic acid content can be obtained. The advantages of this method are: 1. Stable reaction with minimal fading; 2. Simple operation; 3. No interference from reducing sugars or other common reducing substances, ensuring good specificity; 4. High sensitivity. This product is for research use only and is not intended for use in diagnostic procedures.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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