DiIC16(3) (Cell Membrane Fluorescent Probe) - BioReagent, Biological Stain, for fluorescence analysis, for microscopy, ≥95% , CAS No.84109-11-5

CAS: 84109-11-5 Cat. No.: D1452693 Summenformel: C₅₅H₈₉ClN₂O₄ Molekulargewicht: 877.76

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%

Synonyms

1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate | DiIC16(3)

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Cell Staining, Fluorescence Analysis

Übersicht

DiIC16(3) is a long-chain carbocyanine dye. Carbocyanine dyes are widely used as lipophilic tracers for labeling cells, organelles, liposomes, viruses, and lipoproteins. Long-chain carbocyanines include DiO (DiOC18(3)), DiI (DiIC18(3)), DiD (DiIC18(5)), and DiR, as well as the dialkylaminostyryl dye DiA (4-Di-16-ASP), which are used to label membranes and other hydrophobic structures. The alkyl substituent of DiIC16(3) (C16) is shorter than that of DiI (C18). They exhibit extremely high extinction coefficients, environment-dependent fluorescence, and short excited-state lifetimes in lipid environments. They are weakly fluorescent in water but highly fluorescent and reasonably photostable when bound to membranes or lipophilic biomolecules. These optical properties make them ideal for plasma membrane staining. Once applied to cells, these dyes diffuse laterally within the plasma membrane, resulting in staining of the entire cell.

DiO, DiI, DiD, and DiR exhibit distinct green, orange, red, and infrared fluorescence, respectively, facilitating multicolor imaging and flow cytometry analysis of live cells. DiO and DiI can be used in standard FITC and TRITC fluorescence channels, respectively. Among them, DiI and its derivatives are the most commonly used because they generally show very low cytotoxicity. In addition, DiI is widely used for the assay of lipoproteins such as LDL and HDL. The lipophilic aminostyryl dye DiA is also commonly used for neuronal tracing.

Specifications

Synonyme

1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate | DiIC16(3)

Spezifikationen & Reinheit

BioReagent, Biological Stain, for fluorescence analysis, for microscopy, ≥95%

Stabilität und Lagerung

Store at -20℃ long term (12 months). Store in the dark. Avoid freeze/thaw cycle.

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

Biological Stain, BioReagent, für die Fluoreszenzanalyse, für die Mikroskopie

Reinheit

≥95%

Namen und Kennungen

• Molekulargewicht: 877.76

Chemische und physikalische Eigenschaften

• Löslichkeit: Solubility in DMSO: 11.67 mg/mL (13.30 mM; dissolution aided by ultrasonication; hygroscopic DMSO significantly affects the solubility of the product. Please use freshly opened DMSO)
• Empfindlichkeit: Light-sensitive; Moisture sensitive