QUALITÄT & REINHEITCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated für empfindliche chromatographische und analytische Workflows, die minimale Basislinieninterferenzen erfordern.
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Lagerung & Versand
Store at -20°C,Avoid repeated freezing and thawing Versand Ice chest + Ice pads. Genaue Spezifikationen finden Sie im chargenspezifischen COA.
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Qualitätsdokumente
SDS, COA, Datenblatt und Spezifikationsblatt stehen zum Download bereit. Chargenspezifische COA über Chargennummernsuche abrufbar.
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Literaturbeleg
In 0 peer-reviewten Publikationen zu Chromatographie, organischer Synthese und Kreuzkupplungsreaktionen zitiert.
Übersicht
Rabbit anti Human DOK7 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
Spezifikationen
Product Name
DOK7 Antibody - Primärer Antikörper, specific to DOK7, Rabbit IgG
Synonyme
DOK 7 antibody | Docking protein 7 antibody | DOK7 antibody | DOK7_HUMAN antibody | Downstream of tyrosine kinase 7 antibody | Protein Dok-7 antibody | CMS10 | CMS1B | FADS3 | C4orf25
Spezifikationen & Reinheit
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Wirtsspezies
Kaninchen
Spezifität
DOK7
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DOK7.
Positive Kontrolle
WB: Mouse brain, Rat brain lysates. IHC: Human brain tissue. ICC/IF: Hep G2 cells.
Konjugation
Unconjugated
Note
Carrier Free, ExactAb™, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Immunogen affinity purified
Konzentration
1.0 mg/mL
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
DOK7 Antibody (Ab100303) - Western Blot All lanes: DOK7 Antibody (Ab100303) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution
Predicted band size: 53 kDa Observed band size: 56 kDa Exposure time: 32.0 s
DOK7 Antibody (Ab100303) - IF/ICC Immunofluorescent analysis of DOK7 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DOK7 Antibody (Ab100303) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DOK7 Antibody (Ab100303) - IHC Immunohistochemical analysis of DOK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DOK7 Antibody (Ab100303) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anwendung
Anwendung
Dilution info
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
WB
1/500-1/1000
Dokumentation
📋 Sicherheitsdatenblatt (SDS)
Umfassendes Dokument zu Gefahren, Handhabung, Lagerung und Einhaltung regulatorischer Vorschriften.
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