E. Coli DNA Gyrase

Cat. No.: E418763

Übersicht

Product Info

Quality Control Tests:

Nuclease contamination: assayed by testing for linear KDNA and linear plasmid DNA formation.  Incubation of 1 µg of catenated KDNA or supercoiled pUC19 DNA for 4 hrs. at 37°C (under gyrase assay conditions and with or without ATP).

Assay Conditions:

Supercoiling assays are carried out using relaxed pBR-322 DNA under conditions specified in the protocol sheets provided with the product.  We also perform assays using kDNA as substrate.  For details on this decatenation/supercoiling assay please refer to our technical literature (link provided above). One unit of gyrase activity will supercoil 0.2 ug of plasmid in 30 min at 37°C.

Included Materials:

A 5X assay uffer and dilution buffer is included with the enzyme.  These are also sold separately as 5X Gyrase Assay Buffer Gyrase Dilution Buffer (please enquire).

Purification of Gyrase A and B Subunits

Description

Highly purified DNA Gyrase is offered as a holoenzyme (contains both A,B subunits). This is an excellent reagent for supercoiling plasmids in vitro or for novel drug screens.

Shipping&storage

The enzyme is shipped on dry ice and should be stored at -70°C. We also recommend that the enzyme be aliquoted after the first thaw (repeated rounds of freeze/thaw may cause loss of activity); the enzyme activity is stable for 1-3 days on ice.

Specifications

Product Name

E. Coli DNA Gyrase

Molekültyp

Kleines Molekül

Lagerung und Versand

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Häufig gestellte Fragen

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.