QUALITÄT & REINHEITBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
Weigh ~37 g of dehydrated EC Broth and dissolve in 1.0 L of purified water. Mix until clear.
Dispense 10 mL into culture tubes fitted with Durham tubes (optional) or dispense bulk into flasks.
Autoclave at 121 °C for 15 minutes. Cool to 44–45 °C if performing thermotolerant coliform enrichment.
Double-strength EC Broth (literature):
Dissolve ~74 g/L. Used when a large inoculum volume is added to maintain final single-strength concentration.
Enrichment and detection (literature):
Inoculate with test sample (or a measured portion of a pre-enrichment). For thermotolerant coliforms, incubate at 44.5 ± 0.2 °C for 18–24 h; for total coliforms, incubate at 35–37 °C.
Positive indication: turbidity and/or gas collected in Durham tube. Proceed to confirmatory testing (e.g., streak onto selective/differential agar, biochemical assays, or molecular confirmation).
Quality controls:
Include positive control strains (e.g., E. coli reference strain) and negative controls per SOPs.
Verify bath/air incubator temperature with a calibrated thermometer placed in a dummy tube containing broth.
Note: Values above are literature guidelines and not item-specific specifications.
Biological Roles
EC Broth itself has no biological function in organisms; it is a formulated culture medium providing nutrients and selectivity for microbial growth in vitro. The biological relevance lies in how its components shape microbial communities during enrichment (literature):
Component roles (typical, literature):
Peptone/tryptose: supplies amino acids, peptides, and growth factors to support bacterial biosynthesis and proliferation.
Lactose: fermentable carbohydrate enabling differentiation/enrichment of lactose-fermenting coliforms.
Bile salts: mimic intestinal bile conditions and select against many Gram-positive and non-enteric bacteria, enriching for enteric coliforms.
Phosphate buffer (K2HPO4/KH2PO4): maintains pH near neutrality, stabilizing enzyme activity during growth.
NaCl: maintains osmotic balance and ionic strength.
Microbiological context (literature):
When incubated at elevated temperatures (≈44–45 °C), EC Broth becomes relatively selective for thermotolerant coliforms, commonly used as proxies for fecal contamination in research and industrial QC settings.
Gas production from lactose (detected via Durham tubes) provides a convenient indication of active fermentation in enrichment cultures.
Note: Specific performance characteristics (e.g., recovery rates, inclusivity/exclusivity) are method- and strain-dependent. Validate in your laboratory with relevant reference strains.
Buffer Applications
This product is not a dedicated buffering reagent; it is a complete culture medium that contains a built-in phosphate buffer sufficient for its intended microbiological purpose.
General/literature guidance:
Typical final pH after preparation is near neutrality (literature value often cited: ~6.9 ± 0.2 at 25 °C after sterilization), maintained by a K2HPO4/KH2PO4 pair.
If additional buffering is required for a particular experimental design, consider using a defined phosphate-buffered system prepared separately. Altering the buffer strength in EC Broth may change selectivity and growth characteristics and should be validated.
Practical notes:
Measure and record pH of the cooled, sterilized broth; adjust only if your method validation allows it.
For electrophoresis, chromatography, or biochemical assays, use dedicated buffering systems; EC Broth’s complex organic content is not suitable for analytical buffer roles.
Item-specific numeric buffer parameters (pKa, buffer capacity): Not specified for this item; refer to CoA/Spec Sheet.
Green Alternatives
While “green chemistry” frameworks target chemicals and reactions, media preparation also offers sustainability levers. Consider the following (general guidance):
Right-sizing batches: prepare only the volume required for the current study to reduce disposal of unused sterile broth.
Energy efficiency: optimize autoclave load and cycle (validated) to minimize energy and water use; use pre-heated feedwater or shared sterilization runs when practical.
Water stewardship: choose water systems with effective recirculation and recovery; avoid excessive rinsing by validating cleaning-in-place for glassware.
Waste minimization: segregate uninoculated, expired medium from biohazard waste; autoclave only what is necessary. Consider chemical disinfection (validated) for certain waste streams to reduce steam energy demand.
Packaging: if available, select bulk packaging to decrease plastic per unit mass and plan for recycling of drums/buckets and secondary packaging.
Alternatives and tradeoffs (literature):
Chromogenic/fluorogenic media may reduce the need for multiple enrichment/confirmation steps, potentially lowering total resource use, but often have higher embodied energy and cost per test.
Ready-to-use sterile broths eliminate autoclaving but increase cold-chain or packaging impacts. Dehydrated media like this product minimize shipping weight and can be prepared on demand.
Document sustainability metrics (e.g., media utilization rate, steam per liter) as part of lab green initiatives.
Pharmaceutical Uses
No therapeutic or clinical use is intended or implied. For research use only (per Product Data).
Environmental and utility monitoring (R&D/QC): enrichment of coliform indicators from process water or non-sterile utilities as part of research studies or method development (outside of GMP release testing unless validated accordingly).
Raw material or in-process studies: evaluation of bioburden reduction steps in feasibility studies, using EC Broth as an enrichment medium for coliform recovery (research phase).
Training and validation: laboratory training on aseptic technique, enrichment workflows, and MPN enumeration in non-GMP settings.
Regulatory note:
EC Broth may be referenced in compendial or standard methods (e.g., APHA, ISO, FDA/BAM) for indicator organism enrichment (literature). However, applicability depends on compliance status of the specific product and your validated method. Confirm suitability, perform growth-promotion testing, and document any deviations.
Formulation/excipient role: none. This product is not an excipient or drug component.
Always adhere to your organization’s quality system; if GMP use is contemplated, obtain and review full specifications and perform qualification per internal procedures.
Physical Properties
Item-specific physico-chemical specifications are not provided for this catalog entry; see the lot CoA/Spec Sheet for authoritative data.
Item-specific (this product):
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Grade: BioReagent (see Quality & Grades tab).
Storage conditions: Room temperature (per Product Data); protect from moisture.
General/literature characteristics for dehydrated EC Broth media (non-spec, for context only):
Physical form: free-flowing, hygroscopic powder; color ranges tan to beige depending on peptone source.
Solubility: readily soluble in purified water to make the working broth; insoluble in organic solvents.
Typical working concentration (literature): single-strength often prepared at ~37 g per liter; double-strength at ~74 g/L (values are typical literature guidance, not product specs).
Typical final pH (literature): ~6.9 ± 0.2 at 25 °C after sterilization.
Density/bulk density: varies with lot and grind; Not specified for this item; refer to CoA/Spec Sheet.
Refractive index, logP, pKa: Not applicable to mixtures.
Solutions are usually clear to slightly opalescent after dissolution and sterilization (literature).
Quality and Grades
Grade: BioReagent (per Product Data). In Aladdin’s usage, BioReagent-grade materials are suitable for common laboratory and life-science workflows, including microbiology, cell culture auxiliaries, and molecular biology support tasks, where consistent performance and low contaminant background are important. This is not equivalent to clinical, GMP, or pharmacopoeial grades.
What BioReagent implies (general):
Controlled raw materials and manufacturing processes intended to reduce lot-to-lot variability in growth performance.
Baseline limits on common impurities that could interfere with biological experiments (e.g., excessive heavy metals, inhibitory residues), though exact numeric specifications are lot-specific.
Typical QC for media includes growth promotion, sterility (pre-sterilization powder may contain background spores; sterility applies to prepared medium), and pH after preparation (literature). Item-specific acceptance ranges: Not specified for this item; refer to CoA/Spec Sheet.
Stabilizers/additives:
No stabilizers are listed in the Product Data. If present, they would be declared on the CoA. Do not assume antibiotics or indicators are included unless explicitly stated.
Best practices:
Record lot numbers in experimental logs to correlate performance.
For critical work, verify pH and clarity after preparation and sterilization and perform a growth-promotion test with relevant strains.
Reaction and Applications
Chemical reaction use is not applicable. EC Broth is intended for microbiological enrichment and selective cultivation in research and quality-control contexts.
Typical research applications (literature context):
Selective enrichment of thermotolerant coliforms and Escherichia coli by incubation at elevated temperature (commonly 44–45 °C, literature). Lactose fermentation with gas production in inverted Durham tubes is often used as an indication for coliforms after enrichment.
Pre-enrichment step prior to downstream biochemical, molecular, or plating confirmation on differential/selective agars.
Monitoring of fecal indicator bacteria in environmental, food, and process water research (non-clinical, research use only).
Rationale for selectivity (literature):
Bile salts suppress many Gram-positive and non-enteric organisms.
Lactose supports fermentation-based indication for coliforms.
Phosphate buffer maintains pH near neutrality during growth.
Practical tips:
Use freshly prepared medium. Validate autoclave cycle to avoid caramelization or excessive Maillard browning that can alter performance.
Include uninoculated controls to check sterility and pH post-sterilization.
For enumeration workflows, pair enrichment with appropriate MPN or membrane filtration schemes per your laboratory SOPs (research only).
Reaction Conditions
Chemical reaction conditions are not applicable. The relevant “conditions” pertain to medium preparation and incubation for microbiological enrichment (literature guidance; not item-specific specifications):
Preparation (typical literature procedure):
Dissolve the dehydrated medium in purified water to the recommended concentration (commonly ~37 g/L for single-strength EC Broth; adjust volume to final). Mix until fully dissolved.
Distribute into tubes/flasks with optional Durham tubes if monitoring gas formation.
Sterilize by autoclaving (e.g., 121 °C for ~15 minutes). Allow to cool to 44–45 °C before inoculation if performing thermotolerance selection.
Incubation (literature context):
Inoculate with test sample and incubate at the temperature specified by your method. For thermotolerant coliform enrichment, 44.5 ± 0.2 °C in a calibrated water bath is common; for total coliform enrichment, 35–37 °C is often used.
Typical incubation time: 18–24 hours; extend per method if needed for slow-growing strains.
Readout:
Turbidity and/or gas in Durham tubes suggests lactose fermentation by coliforms; follow with confirmatory tests (biochemical, plating, or molecular) as per SOPs.
Always validate conditions in your laboratory and refer to authoritative method documents and the product CoA.
Safety and Handling
Hazard and GHS details are not provided in the Product Data. Always consult the product SDS for authoritative safety information.
GHS (this item):
Signal word, hazard statements, pictograms, classification: Not specified for this item; refer to SDS/CoA.
General laboratory safety guidance for dehydrated microbiological media (literature/best practice):
PPE: wear lab coat, safety glasses, and appropriate gloves. Avoid inhalation of dust; handle powders in a well-ventilated area or fume hood.
Incompatibilities: prevent exposure to moisture (caking, degradation). Store away from strong oxidizers and strong acids/bases.
Thermal hazards: hot media can cause burns; use heat-resistant gloves when removing from autoclave/water bath.
Microbiological risk: once inoculated, all cultures and spent media should be treated as biohazardous. Follow BSL-1/2 practices as appropriate to the organism handled. Decontaminate by autoclaving before disposal.
Dust control: use gentle pouring and antistatic measures to minimize airborne particulates.
First aid (general): if inhaled—move to fresh air; eye/skin contact—rinse with water; if ingested—rinse mouth. Seek medical attention per SDS guidance.
Shipping/storage:
Shipped at ambient temperature (per Product Data). Store tightly closed at room temperature in a dry place; use desiccant where possible to protect from humidity.
Solvent Selection
This product is a dehydrated microbiological medium formulated to be reconstituted in water. Organic solvent selection is not applicable.
Practical guidance:
Use purified/deionized water of consistent quality (e.g., ASTM Type II or better for microbiological media) to minimize variability from ions or organics.
Avoid high-resistivity RO water that may be incompatible with some autoclaves if silica carryover is an issue; rinse glassware thoroughly to remove detergents that can inhibit bacterial growth.
Do not substitute buffers or salts beyond the intended formulation; the medium already contains a phosphate buffering system (literature) set near neutrality.
Comparison (literature context):
EC Broth versus LB/TSB: EC is selective (contains bile salts; lactose fermentation readout), whereas LB/TSB are non-selective, richer for general growth.
EC Broth versus m-ColiBlue or chromogenic media: EC is a broth enrichment, typically used prior to confirmatory tests; chromogenic/fluorogenic media provide direct visual indicators but are more specialized.
If gelling is required for plate-based work, use an agar-based selective medium (e.g., EC agar variants); adding agar to EC Broth changes performance and should be validated.
Storage and Reconstitution
Storage (this item):
Room temperature (per Product Data). Keep container tightly closed, in a dry environment, protected from humidity and excessive heat. Use desiccant if available. Shipped under normal conditions.
Shelf life and appearance:
Not specified for this item; refer to CoA/Spec Sheet. Inspect for clumping or discoloration that may indicate moisture ingress or overheating during storage.
Reconstitution and sterilization (literature guidance):
Reconstitute in purified water at the desired concentration (e.g., ~37 g/L for single-strength). Mix thoroughly until dissolved.
Distribute into suitable vessels; include Durham tubes if gas detection is desired.
Sterilize by autoclaving (e.g., 121 °C for 15 minutes). Do not over-sterilize to avoid nutrient degradation or excessive browning.
After sterilization, allow to cool to the working temperature. If not used immediately, store prepared broth at 2–8 °C and use promptly (holding time should be minimized; define per SOP). Holding-time/expiry of prepared media: Not specified for this item; refer to CoA/Spec Sheet.
Freeze–thaw:
Not applicable to dehydrated powder. Avoid freezing prepared broth unless validated, as precipitation or performance shifts may occur.
Always consult the product CoA/SDS and your lab’s SOPs for definitive instructions.
Structure and Identity
This product is a complex, dehydrated microbiological growth medium (EC Broth), not a single defined chemical compound. Therefore, single-molecule identifiers do not apply.
Product type: Complex nutrient medium (selective enrichment for coliforms, especially thermotolerant E. coli, literature)
Composition nature: Mixture of peptones/tryptose, lactose, bile salts/oxgall, phosphate buffers, sodium chloride (typical components per literature; exact composition and lot-specific specification may vary by manufacturer)
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Heterogeneous blend of organic and inorganic solids that, when dissolved in water, forms a nutrient-rich, mildly selective, phosphate-buffered broth supporting growth of coliform bacteria under prescribed incubation conditions (literature).
Synthetic Utility
Not applicable. EC Broth is a microbiological culture medium and does not serve as a reagent for organic synthesis or as a building block in chemical transformations.
If your project involves chemical synthesis related to microbiology (e.g., labeling, quench, or extraction from cultures), select reagents and solvents according to the needs of the chemical reaction, and treat the culture medium as a complex aqueous matrix potentially containing proteins, peptides, carbohydrates, and salts that can interfere with downstream chemistry.
Target Specificity
This section typically applies to antibodies or affinity reagents and is not applicable to a culture medium.
Context (literature): EC Broth is formulated to be selective for coliforms, especially thermotolerant coliforms including Escherichia coli, through the combined effects of bile salts, lactose fermentation, and incubation at elevated temperature. Inclusivity/exclusivity profiles are method- and strain-dependent and should be verified with appropriate control organisms.
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