EGFR Mouse mAb (AF594) - Primärer Antikörper, specific to EGFR

Cat. No.: Ab217690

GRADE & PURITY ExactAb™, Validated, 5 μL/test

Application

Flow Cytometry

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2b

Clone Number

EGFR.1

Conjugation

AF594

Übersicht

Mouse anti Human EGFR Antibody, Monoclonal (EGFR.1), could be used for Flow Cytometry.

Specifications

Product Name

EGFR Mouse mAb (AF594) - Primärer Antikörper, specific to EGFR

Synonyme

Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody | Cell growth inhibiting protein 40 antibody | Cell proliferation inducing protein 61 antibody | EGF R antibody | EGFR antibody | EGFR_HUMAN antibody | Epidermal growth factor recepto

Spezifikationen & Reinheit

ExactAb™, Validated, 5 μL/test

Biochemische und physiologische Mechanismen

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,

Wirtsspezies

Maus

Spezifität

EGFR

Immunogen

A431 cultured cells

Positive Kontrolle

Flow Cytometry: A431

Konjugation

AF594

Anregung (nm)

Yellow-Green Laser(594nm)

Emission (nm)

618nm

Ex/Em(nm)

Ex:590nm;Em:618nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

5 μL/test

Product Line

biological_products

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: EGFR Mouse mAb (AF594) (Ab217690)

EGFR Mouse mAb (AF594) (Ab217690) - Flow Cytometry
Flow cytometry analysis of EGFR expression by A431 cells (red) compared to an isotype control (blue). Cells were stained with EGFR Mouse mAb (AF594) (Ab217690) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF594) (Ab190247). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Zugehörige Ziele (menschlich)

EGFR Tclin Epidermal growth factor receptor (0 Activities)

Discover Target: EGFR

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (EGFR Mouse mAb (AF594)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.