GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A,
Wirtsspezies
Kaninchen
Spezifität
EIF3D
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF3D.
Positive Kontrolle
WB: HeLa cell lysate. IHC: Human lung cancer tissue.
Konjugation
Unconjugated
Note
Carrier Free, ExactAb™, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Immunogen affinity purified
Konzentration
1.0 mg/mL
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
EIF3D Antibody (Ab101319) - Western Blot All lanes: EIF3D Antibody (Ab101319) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 65 kDa Exposure time: 100.0 s
EIF3D Antibody (Ab101319) - IHC Immunohistochemical analysis of EIF3D staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the EIF3D Antibody (Ab101319) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anwendung
Anwendung
Dilution info
IHC
1/100 - 1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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