eIF3l Antibody - Primärer Antikörper, specific to EIF3L, Rabbit IgG

Cat. No.: Ab101338

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Affe, Allerdings, Huhn, Kuh, Maus, Mensch, Rat, Schwein

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human eIF3l Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

eIF3l Antibody - Primärer Antikörper, specific to EIF3L, Rabbit IgG

Synonyme

eIEF associated protein HSPC021 antibody | EIF3EIP antibody | eif3l antibody | EIF3L_HUMAN antibody | EIF3S11 antibody | EIF3S6IP antibody | Eukaryotic translation initiation factor 3 subunit 6 interacting antibody | Eukaryotic translation initiation fact

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Biochemische und physiologische Mechanismen

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, e

Wirtsspezies

Kaninchen

Spezifität

EIF3L

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EIF3L.

Positive Kontrolle

WB: HEK293, HeLa, Jurkat, Hep G2 and NIH/3T3 cell lysates. IHC: Human Pancreas tissue. IF/ICC: HEK293 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Immunogen affinity purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: eIF3l Antibody (Ab101338)

eIF3l Antibody (Ab101338) - Western Blot
All lanes: eIF3l Antibody (Ab101338) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 62 kDa
Exposure time: 11.4 s

IHC: eIF3l Antibody (Ab101338)

eIF3l Antibody (Ab101338) - IHC
Immunohistochemical analysis of EIF3L staining in human Pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with eIF3l Antibody (Ab101338) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: eIF3l Antibody (Ab101338)

eIF3l Antibody (Ab101338) - IF/ICC
Immunofluorescent analysis of EIF3L staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with eIF3l Antibody (Ab101338) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Anwendung

Anwendung

Dilution info

IHC

1/100 - 1/200

IF/ICC

1/100-1/500

WB

1/500-1/1000

Zugehörige Ziele (menschlich)

EIF3L Tbio Eukaryotic translation initiation factor 3 subunit L (0 Activities)

Discover Target: EIF3L

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (eIF3l Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.