Eosinophil Staining Solution - BioReagent,Biological Stain,for microscopy

Cat. No.: E1511531

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Synonyms

Eosinophil Stain

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Übersicht

Eosinophils are one type of inflammatory cellular component and mostly function in allergic diseases. The cytoplasm of eosinophils contains secondary granules, which mainly comprise basic proteins, such as eosinophil cationic protein, eosinophil-derived neurotoxin, and eosinophil peroxidase.

Eosinophil Staining Solution consists of Carbol 2R Staining Solution and Hematoxylin Staining Solution. The cytoplasm of eosinophils is stained pink, and the nucleus is stained blue. This reagent is for research use only and not for clinical diagnosis or other purposes.

Product Components and Storage Conditions:

E1511531	Component	2×100mL	Storage
E1511531A	Carbol 2R Stain	100mL	RT. Store in the dark.
E1511531B	Hematoxylin Stain	100mL	RT

 Protocol (For Reference Only):

1. Tissue fixation (10% neutral formalin fixative is recommended), followed by routine dehydration, embedding, and dewaxing to water.

2. Stain in Carbol 2R Stain for 15–20 min, then rinse with distilled water.

3. Stain in Hematoxylin Stain for 3–8 min, then rinse with distilled water.

4. Dehydrate in graded ethanol, clear routinely, and mount with neutral balsam.

Staining Results:

Cytoplasm of eosinophils: Pink

Nuclei: Blue

Precautions: 

1. If differentiation is unsatisfactory, 0.5% hydrochloric acid alcohol may be used for differentiation to remove background.

2. Can also be used for staining cell smears, but the staining time should be shortened accordingly; fix with 4% paraformaldehyde solution.

3. Please use the reagent as soon as possible after opening to avoid affecting experimental results.

4. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Synonyme

Eosinophil Stain

Spezifikationen & Reinheit

BioReagent,Biological Stain,for microscopy

Stabilität und Lagerung

Store at room temperature long term (12 months). Store in the dark.

Storage

Protected from light,Room temperature

Verschickt in

Normal

Note

Biological Stain, BioReagent, für die Mikroskopie

Product Line

Biologische Produkte

Häufig gestellte Fragen

What does Biological Stain mean?

Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.