Epothilone A - 10mM in DMSO , CAS No.152044-53-6

CAS: 152044-53-6 Cat. No.: E409291 Molecular Weight: 493.66

GRADE & PURITY 10mM in DMSO

Synonyms

(1S,3S,7S,10R,11S,12S,16R,E)-7,11-dihydroxy-8,8,10,12-tetramethyl-3-(1-(2-methylthiazol-4-yl)prop-1-en-2-yl)-4,17-dioxa-bicyclo[14.1.0]heptadecane-5,9-dione

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Epothilone A Epothilone A is a paclitaxel-like microtubule -stabilizing agent with EC 0.01 of 2 μM.
In vitro

Epothilone A, discovered from the myxobacterium Sorangium cellulosum, is a Taxol-like microtubule-stabilizing agent that induces tubulin polymerization, leading to cell cycle arrest at the G2-M transition, cytotoxicity, and apoptosis.Epothilone A potently inhibits cell proliferation in HCT116 cells, with IC50 of 4.4 nM. Epothilone A also displays cytotoxicity in KB3-1, KBV-1, Hela, and Hs578T cells, with IC50 values ranging from 13 nM to 160 nM. Epothilone A is more water soluble than Taxol and competes with Taxol in binding with microtubules, with IC50 of 2.3 μM. However, both Epothilone A and Taxol don\'t share a common pharmacophore and exploits the tubulin-binding pocket uniquely and independently. Recently, it is found that microbiological transformation of Epothilone A by Aspergillus niger AS 3.739 yields several metabolites that is also toxic to MCF-7 cells, but with much higher IC50 values.

In vivo

Cell Data

cell lines：A431, MO7e, HUVEC and NHDF cells

Concentrations：0–2 μM

Incubation Time：24 hours for mitotic arrest or 72 hours for cytotoxicity

Powder Purity:≥98%

Specifications

Synonyms

(1S, 3S, 7S, 10R, 11S, 12S, 16R, E)-7, 11-dihydroxy-8, 8, 10, 12-tetramethyl-3-(1-(2-methylthiazol-4-yl)prop-1-en-2-yl)-4, 17-dioxa-bicyclo[14.1.0]heptadecane-5, 9-dione

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

Epothilone A is a paclitaxel-like microtubule-stabilizing agent with EC_0.01 of 2 μM.

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Isomeric SMILES: C[C@H]1CCC[C@@H]2[C@@H](O2)C[C@H](OC(=O)C[C@@H](C(C(=O)[C@@H]([C@H]1O)C)(C)C)O)/C(=C/C3=CSC(=N3)C)/C
• WGK Germany: 3
• Molecular Weight: 493.66
• Reaxy-Rn: 26220988
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=26220988&ln=