≥98% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C,Desiccated Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide , highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K + channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.
In Vitro
1. Preparation of ER-Tracker working solution 1.1 Preparation of the stock solution Dissolve 100 ug ER-Tracker in 128 μL DMSO to obtain 1 mM of stock solution. Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of ER-Tracker working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 100 nM-1 μM of working solution. Note: Please adjust the concentration of ER-Tracker working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells (6-well plate) a. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 /mL. b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes. c. Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes. d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry. Note: If detection by flow cytometry, cells need to be resuspended before staining. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
| Kanonisches Lächeln | O=C(NS(=O)(C1=CC=C(CCNC(C2=C(OC)C(NC(CCC3=CC=C4C=C5C(C)=CC(C)=[N]5[B+3]([F-])([N-]43)[F-])=O)=CC(Cl)=C2)=O)C=C1)=O)NC6CCCCC6 |
|---|---|
| Molekulargewicht | 783.09 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Datum | Artikel |
|---|---|---|---|
 E2527686 | Certificate of Analysis | May 16, 2025 | E651376 |
| E2527687 | Certificate of Analysis | May 16, 2025 | E651376 |
| E2527692 | Certificate of Analysis | May 16, 2025 | E651376 |
| E2527707 | Certificate of Analysis | May 16, 2025 | E651376 |
| E2527710 | Certificate of Analysis | May 16, 2025 | E651376 |
| Löslichkeit | DMSO : 50 mg/mL (63.85 mM; Need ultrasonic) |
|---|---|
| Empfindlichkeit | Moisture sensitive |
| 1. Pengkai Ma, Ziqi Jing, Xue Wang, Xiaoya Liu, Zirui Tan, Yujie Zhang, Zhijun Wang. (2025) Carrier-free multi-components self-delivery nanocomplex for tumor synergistic therapy. International Journal of Pharmaceutics-X, [PMID:41323844] [10.1016/j.ijpx.2025.100443] |
