Estradiol Benzoate - 10mM in DMSO , CAS No.50-50-0

CAS: 50-50-0 Cat. No.: E408424 Molecular Weight: 376.49 Beilstein Registry Number: 3107526 EC Number: 200-043-7

GRADE & PURITY 10mM in DMSO

Synonyms

Estra-1,3,5(10)-triene-3,17-diol (17β)-, 3-benzoate

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Estradiol benzoate is an estradiol analog which binds to the human, murine and chickenERαwithIC50of 22-28 nM.
In vitro

Estradiol benzoate is asynthetic ester, specifically the 3-benzoyl ester, of the natural estrogen, estradiol. Estrogens direct the development of the female genotype in embryogenesis and at puberty. Estradiol is the major estrogen secreted by the premenopausal ovary. Estradiol benzoate is an estradiol analog which contains a benzyl ester at the C-3 position and is often used in combination with a progestin to induce estrus in domestic livestock. This compound binds to the human and murine estrogen receptor α (ERα), and chicken ER with IC50 values in the range of 22-28 nM. This reflects a 6-10 fold reduction in binding affinity compared to estradiol.

In vivo

Cell Data

cell lines：

Concentrations：

Incubation Time：

Powder Purity:≥97%

Specifications

Synonyms

Estra-1, 3, 5(10)-triene-3, 17-diol (17β)-, 3-benzoate

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

Estradiol benzoate is an estradiol analog which binds to the human, murine and chicken ERα with IC50 of 22-28 nM.

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Isomeric SMILES: C[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2O)CCC4=C3C=CC(=C4)OC(=O)C5=CC=CC=C5
• WGK Germany: 3
• RTECS: KG4050000
• Molecular Weight: 376.49
• Beilstein: 3107526
• Reaxy-Rn: 2063518
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=2063518&ln=

Associated Targets(Human)

ESR1 Tclin Estrogen receptor (2 Activities)

Discover Target: ESR1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro Ethanol: mg/mL
• Melt Point(°C): 191-198°C

References

1. Dong Xiaoyu, Xu Le, Wang Shuang, Jiao Xue, Yan Shumin, Huang Yufei, Yuan Ming, Wang Guoyun.  (2023)  Endometrial stromal cell autophagy-dependent ferroptosis caused by iron overload in ovarian endometriosis is inhibited by the ATF4-xCT pathway.  MOLECULAR HUMAN REPRODUCTION,      [PMID:38113413] [10.1093/molehr/gaad046]

2. Qiaomei Zheng, Ping Wu, Yanjing Zhao, Jinhua Wang, Shaozhan Chen, Lihong Chen.  (2023)  Features of peritoneal dendritic cells in the development of endometriosis.  Reproductive Biology and Endocrinology,  21  (1): (1-8).  [PMID:36639763] [10.1186/s12958-023-01058-w]

3. Zhang Yu, Su Na, Liu Weiyi, Wang Qingqing, Sun Jianguo, Peng Ying.  (2021)  Metabolomics Study of Guizhi Fuling Capsules in Rats With Cold Coagulation Dysmenorrhea.  Frontiers in Pharmacology,      [PMID:34776980] [10.3389/fphar.2021.764904]

4. Li Qiuju, Yuan Ming, Jiao Xue, Huang Yufei, Li Jing, Li Dong, Ji Miaomiao, Wang Guoyun.  (2021)  M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis.  Frontiers in Immunology,      [PMID:34354711] [10.3389/fimmu.2021.707784]

5. Xiao Qing, Liu Qi-Meng, Jiang Ru-Chao, Chen Kai-Feng, Zhu Xiang, Ma Lei, Li Wei-Xi, He Fei, Huang Jun-Jun.  (2021)  Piperazine-Derived α1D/1A Antagonist 1- Benzyl-N- (3-(4- (2-Methoxyphenyl) Piperazine-1-yl) Propyl) -1H- Indole-2- Carboxamide Induces Apoptosis in Benign Prostatic Hyperplasia Independently of α1-Adrenoceptor Blocking.  Frontiers in Pharmacology,      [PMID:33584271] [10.3389/fphar.2020.594038]

6. Zhu Jialei, Tang Jing.  (2020)  LncRNA Gm14205 induces astrocytic NLRP3 inflammasome activation via inhibiting oxytocin receptor in postpartum depression.  BIOSCIENCE REPORTS,  40  (8):   [PMID:32706026] [10.1042/BSR20200672]

7. Qi-meng Liu, Qing Xiao, Xiang Zhu, Kai-feng Chen, Xia-wen Liu, Ru-chao Jiang, Dan Wu, Jun-min Shi, Li-jun Dai, Jun-jun Huang.  (2019)  Inhibitory effect of α1D/1A antagonist 2-(1H-indol-3-yl)-N-[3-(4-(2-methoxyphenyl) piperazinyl) propyl] acetamide on estrogen/androgen-induced rat benign prostatic hyperplasia model in vivo.  EUROPEAN JOURNAL OF PHARMACOLOGY,      [PMID:31756334] [10.1016/j.ejphar.2019.172817]

8. Guotao Chen, Jianhuai Chen, Baibing Yang, Wen Yu, Yun Chen, Yutian Dai.  (2018)  Dopamine D2 receptors in the basolateral amygdala modulate erectile function in a rat model of nonorganic erectile dysfunction.  ANDROLOGIA,  51  (1): (e13160).  [PMID:30276840] [10.1111/and.13160]

9. Yuan Ming, Li Dong, An Min, Li Qiuju, Zhang Lu, Wang Guoyun.  (2016)  Rediscovering peritoneal macrophages in a murine endometriosis model.  HUMAN REPRODUCTION,  32  (1): (94-102).  [PMID:27816922] [10.1093/humrep/dew274]

10. Jun-Jun Huang, Yi Cai, Yan-Zhen Yi, Min-Yi Huang, Liu Zhu, Fei He, Xia-Wen Liu, Bi-Yun Huang, Mu Yuan.  (2016)  Pharmaceutical evaluation of naftopidil enantiomers: Rat functional assays in vitro and estrogen/androgen induced rat benign prostatic hyperplasia model in vivo.  EUROPEAN JOURNAL OF PHARMACOLOGY,      [PMID:27615445] [10.1016/j.ejphar.2016.09.009]

11. Mingyu Chang, Jingcheng Lv, Lianhui Sun, Aihong Chen, Fengbo Zhang, Guangjian Fan, Zhanliang Liu, Jun Li, Hongliang Shen, Mingjun Shi, Yinong Niu, Ye Tian, Xijian Liu, Boyu Yang.  (2025)  Cu–Mn Bimetallic Mesoporous Silica Nanosonosensitizers Enable Oxeiptosis-Mediated Sonodynamic Therapy for Ultra-Minimally Invasive Treatment of Benign Prostatic Hyperplasia.  ACS Applied Materials & Interfaces,      [PMID:40911431] [10.1021/acsami.5c12846]

12. Zeng Xinliu, Yue Zhang, Gao Ying, Jiang Guosong, Zeng Fuqing, Shao Ying, Huang Jiayu, Yin Minuo, Li Yajie.  (2018)  NR4A1 is Involved in Fibrogenesis in Ovarian Endometriosis.  CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,  46  (3): (1078-1090).  [PMID:29669342] [10.1159/000488838]

13. Chen Guotao, Yang Baibing, Chen Jianhuai, Zhu Leilei, Jiang Hesong, Yu Wen, Zang Fengchao, Chen Yun, Dai Yutian.  (2017)  Changes in Male Rat Sexual Behavior and Brain Activity Revealed by Functional Magnetic Resonance Imaging in Response to Chronic Mild Stress.  Journal of Sexual Medicine,  15  (2): (136-147).  [PMID:29275048] [10.1016/j.jsxm.2017.11.221]

14. Zhou Si Yun, Song Zhihua, Tian Xuelian, Xia Min, Hu Rongjing, Luo Mei.  (2024)  Rosmarinic acid activates the Ras/Raf/MEK/ERK signaling pathway to regulate CD8+ T cells and autophagy to clear Chlamydia trachomatis in reproductive tract-infected mice.  MOLECULAR IMMUNOLOGY,      [PMID:38820902] [10.1016/j.molimm.2024.05.007]

15. Yue Wei, Tingyun Ma, Hanxue Wang, Jianguo Xing, Yuwen Wang, Zhengyi Gu, Dandan Mu, Qiang Yin, Xuemei Cheng, Changhong Wang.  (2018)  Extracts of compound Muniziqi granule suppressed uterus contraction and ameliorated oxytocin-induced primary dysmenorrhea.  JOURNAL OF ETHNOPHARMACOLOGY,      [PMID:29783015] [10.1016/j.jep.2018.05.024]

16. Yan Xu, Jingxi Zhang, Mengyue Zhang, Jingyu Sun, Zhengdao Chen, Qingsheng Liang, Shaohong Chen, Chuanyin Hu, Yun-Tao Zhao.  (2026)  Cornus officinalis Siebold & Zucc. ethanol extract alleviates primary dysmenorrhea via anti-inflammatory and spasmolytic effects: network pharmacology and experimental verification.  FITOTERAPIA,      [PMID:] [10.1016/j.fitote.2026.107176]