Exosome Assay Kit (ELISA Sandwich Method) - BioReagent, sterile, high purity

Cat. No.: E778166

GRADE & PURITY BioReagent, Sterile

Übersicht

This product is an exosome qualitative and quantitative detection kit based on the sandwich ELISA method. It adopts high-performance CD9 capture antibody and CD81 detection antibody to sensitively detect CD9 and CD81 targets co-expressed on the surface of exosomes. Compatible with various samples such as human body fluids and cell culture media, this kit enables rapid and accurate quantification of exosomes in raw samples.

E778166	Component	24T	48T	96T	Storage
E778166A	Capture Antibody-Coated Microplate	24 wells	48 wells	96 wells	2-8°C
E778166B	Exosome Standard S1	1 mL	1 mL	1 mL	-20°C
E778166C	Enzyme Conjugate	2.5 mL	5 mL	10 mL	2-8°C
E778166D	Chromogenic Solution A	1.5 mL	3 mL	6 mL	2-8°C. Store in the dark.
E778166E	Chromogenic Solution B	1.5 mL	3 mL	6 mL	2-8°C. Store in the dark.
E778166F	Stop Solution	1.5 mL	3 mL	6 mL	2-8°C
E778166G	Concentrated Wash Buffer (20×)	7.5 mL	15 mL	30 mL	2-8°C
E778166H	Assay Diluent	1.25 mL	2.5 mL	5 mL	2-8°C
E778166I	Exosome Standard S2	1 mL	1 mL	1 mL	-20°C
E778166J	Exosome Standard S3	1 mL	1 mL	1 mL	-20°C
E778166K	Exosome Standard S4	1 mL	1 mL	1 mL	-20°C
E778166L	Exosome Standard S5	1 mL	1 mL	1 mL	-20°C

Product Features:

Wide Application: Compatible with various sample types including cell culture supernatant, urine, serum, etc.

Easy Operation: Only a microplate reader with 450 nm wavelength is required; no additional equipment is needed.

Excellent Stability and Reproducibility: This kit adopts CD9/CD81 fusion protein standards instead of native exosomes, which are unstable and hard to preserve, to guarantee superior stability and repeatability.

Exosome Integrity Assay: Enables rapid evaluation of exosome integrity.

Product Principle:

Test Procedure:

1. Pre-test Preparation

① All reagents shall be equilibrated to room temperature for at least 15 minutes before use.

② Dilute the 20× Concentrated Wash Buffer with distilled or deionized water to prepare 1× working solution, mix thoroughly for later use. (The kit is supplied with assay diluent, which can be used to dilute high-concentration standards as required.)

2. Operating Steps

① Take the equilibrated microplate strips and reagents, and fully shake all reagents evenly.

② Add serially diluted Exosome Standards and test samples into respective wells of the microplate (100 μL per well).

③ Seal the reaction wells with plate sealer, and incubate at a constant temperature of 37 °C away from light for 60 min.

④ After incubation, wash wells with wash working solution 3 times, 200–250 μL per well each time. Blot dry on absorbent filter paper after washing.

⑤ Add 100 μL Enzyme Conjugate to each well, seal the plate, and incubate at 37 °C in the dark for 60 min.

⑥ Repeat the washing procedure 3 times as described above, then blot dry completely.

⑦ Prepare chromogenic substrate working solution: mix Chromogenic Solution A and Solution B at an equal volume ratio. Use the mixed substrate within 2 minutes after preparation.

⑧ Pipette 100 μL freshly prepared chromogenic substrate working solution into each well, and incubate at 37 °C protected from light for 30 min.

⑨ Add 50 μL Stop Solution to each well, mix gently, and measure OD₄₅₀ values immediately (within 3 min). Plot an XY linear calibration curve.

3. Calibration Protocol

XY linear fitting is adopted, with a mandatory requirement that the coefficient of determination R² ≥ 0.99. A full set of calibrants must be tested in each independent experiment to generate the calibration curve.

校准点	S1	S2	S3	S4	S5
Concentration (ng/mL)	0	7.816	15.625	31.25	45
OD₄₅₀	0.0737	0.3074	0.5183	0.8618	1.2945

                                                                Exosome Standard Calibration Curve

                                                                                Concentration (ng/mL)

Note: This figure is for reference only. The concentration of exosomes in samples shall be calculated based on the standard curve generated from standards tested in the same assay run.

Precautions and Disclaimer

1. This kit shall be stored away from light.

2. This product is intended solely for scientific research use by professionals. It must not be used for clinical diagnosis, clinical treatment, food or pharmaceutical applications.     

Specifications

Synonyme

Exosome ELISA Kit

Spezifikationen & Reinheit

BioReagent, sterile

Note

BioReagent, Sterile

Empfindlichkeit

Light-sensitive

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Store at -20°C

Verschickt in

Wet ice,Ice chest + Ice pads

Stabilität und Lagerung

Each component has a shelf life of 12 months under corresponding storage conditions.

Contents & Storage

Storage Unit No.	Storage Unit Name	Component No.	Component Name	Appearance	24T	48T	96T	Storage
E778166WT	Exosome Assay Kit (ELISA Sandwich Method) (2-8℃ Components)	E778166A	Capture Antibody-Coated Microplat	/	24T	48T	96T	2-8℃. Store in the dark.
		E778166C	Enzyme Conjugate	Liquid	2.5 mL	5 mL	10 mL	2-8℃.
		E778166D	Chromogenic Solution A	Liquid	1.5 mL	3 mL	6 mL	2-8°C. Store in the dark.
		E778166E	Chromogenic Solution B	Liquid	1.5 mL	3 mL	6 mL	2-8°C. Store in the dark.
		E778166F	Stop Solution	Liquid	1.5 mL	3 mL	6 mL	2-8℃.
		E778166G	Concentrated Wash Buffer (20×)	Liquid	7.5 mL	15 mL	30 mL	2-8℃.
		E778166H	Assay Diluent	Liquid	1.25 mL	2.5 mL	5 mL	2-8℃.
E778166DT	Exosome Assay Kit (ELISA Sandwich Method) (-20℃ Components)	E778166B	Exosome Standard S1	Liquid	1 mL	1 mL	1 mL	-20℃.
		E778166I	Exosome Standard S2	Liquid	1 mL	1 mL	1 mL	-20℃.
		E778166J	Exosome Standard S3	Liquid	1 mL	1 mL	1 mL	-20℃.
		E778166K	Exosome Standard S4	Liquid	1 mL	1 mL	1 mL	-20℃.
		E778166L	Exosome Standard S5	Liquid	1 mL	1 mL	1 mL	-20℃.

Notes: Each component is individually packaged according to its storage temperature (2‑8 °C / -20 °C) upon shipment, with differentiated temperature‑controlled transportation to guarantee product performance. Orders containing materials requiring multiple temperature zones may be delivered in separate batches; please refer to the actual received goods. Upon receipt, store components separately at the temperatures specified in the component list. The shelf‑life is 12 months from the date of manufacture.