A Broth (Powder)

Directions per Liter:

Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Ammonium Sulfate	1
Potassium Phosphate Dibasic	10.5
Potassium Phosphate Mono	4.5
Sodium Citrate	0.5
	Total: 16.5g/Liter
	500g makes 32 liters

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.