Protocols

Acrylamide (30%) Recipe

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

1.Dissolve 290g of acrylamide and 10g of N,N'-methylbisacrylamide in 600ml of H2O. Heating may be necessary to dissolve the acrylamide.

2.Adjust the volume to 1L with H2O.

3.Sterilize the solution by filtration (0.45 micron pore size).

4.Check the pH (should be 7.0 or less).

5.Store in dark bottles at room temperature.

Caution: Acrylamide is a neurotoxin. Use extreme caution when handling anything with acrylamide and bisacrylamide. Protective equipment should be worn.

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Acrylamide (30%) Recipe" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/acrylamide-30-percent-recipe-en.html

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