Bismuth Sulfite Agar (Powder)

Directions per Liter:

Dissolve 53grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).

Component	g/liter
Casein Digest Peptone	5
Sodium Chloride	5
Peptic Digest of Animal	5
Beef Extract	5
Disodium Phosphate	5
Ferrous Sulfate	0.3
Bismuth Sulfite Indicator	8
Brilliant Green	0.025
Agar	20
	Total: 53g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.