Blood Agar Base, pH 7.4 (Powder)

Directions per Liter:

Dissolve 40grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).

Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium.

Cool to 45-50°C. Dispense into Petri dishes or tubes

Component	g/liter
Peptic Digest of Animal Tissue	10
Sodium Chloride	5
Heart Infusion Solids	10
Agar	15
	Total: 40g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.