Casman Medium Base (Powder)

Directions per Liter:

Dissolve 43g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave at 121ºC for 15 minutes. After cooling medium to 50ºC, aseptically add 5% sterile blood and 0.15% sterile water-lysed blood solution (one part blood to three parts water).

Component	g/liter
Enzymatic Digest of Casein	5
Enzymatic Digest of Animal Tissue	5
Yeast Enriched Peptone	10
Beef Extract	3
Niacinamide	0.05
p-Aminobenzoic Acid	0.05
Dextrose	0.5
Corn Starch	1
Sodium Chloride	5
Agar	13.5
	Total: 43g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.