Microphotography is a method of taking pictures through a microscope. It is one of the indispensable means for scientific research, experience exchange and arbitration negotiation in animal and plant quarantine work. To achieve good micrographic effect, the most important thing is to have good microscope equipment and good microscope operation technology.
Operation method
basic program
Principle
Microphotography is the use of a photographic device to capture images of objects observed in the field of view of a microscope. In biomedicine, it is mainly used to record the microscopic morphology of normal or diseased cells.
Materials and Instruments
Human Chromosome Specimen Move I. Preparation before shooting For more product details, please visit Aladdin Scientific website.
Developing solutions Stopping agents Fixing solutions
Microscopes Cameras Microscope Illuminators Automatic Exposure Controllers Darkroom Equipment
1. optical path coaxial Make the optical axis and the beam in the same axis.
2. Köhler's illumination so that the observation of the field of view is hindered by uniform and adequate illumination; to prevent stray light on the camera system has an impact; so that the object is not subject to heat, the image is clear.
3. reasonable combination of objective and eyepiece According to the different specimens and requirements of micrography, the combination of objective and eyepiece has certain specifications, such as: shooting human chromosome images, the use of high-resolution 100 times the oil lens, with 10 times the eyepiece, you can get a good resolution of the ideal magnification of the image.
4. Adjust the field of view diaphragm and aperture diaphragm so that the two and the numerical aperture of the objective lens used (N. A. Mirror mouth rate) is equal to the highest resolution; field of view diaphragm once adjusted, do not move, and the aperture diaphragm can be with the replacement of the numerical aperture of the objective lens, make the appropriate adjustments.
5. In general, the size of the aperture diaphragm adjusted to equal to the phase should be object lens aperture like 2 / 3 appropriate, despite the loss of a small amount of resolving power, but can improve the image of the contrast, depth of focus and clarity.
6. In conclusion, the above practice is to adjust the microscope to the best condition.
Second, the shooting program
1. Open the back cover of the camera and install the film in the camera.
2. Turn on the power switch of the microscope lighting unit and adjust the brightness to the appropriate level.
3. Adjust the focusing ring of the camera eyepiece until the tic-tac-toe line is clear, depending on your eyesight.
4. Place a slide of the specimen to be photographed on the stage. Select the nucleus or cell structure to be photographed under low magnification, then switch to the oil lens to focus on the details of the specimen and prepare for photographing.
5. Press the exposure button. The exposure button lights up to indicate that the exposure is in progress and goes out to indicate that the exposure is complete.Third, black and white film development
1. Developing procedure
(1) the film into the developing tank (do not stick together) first rinse with water to eliminate air bubbles. Then pour out the water, which is conducive to the rapid homogenization of the developing fluid.
(2) from the top of the tank into the mouth of the pre-prepared about 250 ml D-72 type development fluid. 20 ℃ development 4 ~ 12 minutes.
(3) Gently shake the canister during development so that the surface of the film is fully affected by the developer, and pour out the developer quickly when development is completed.
(4) Immediately inject 250 ml of pre-prepared SB-I type stopping solution, stopping time is 10 seconds, pour out the stopping solution, then add 250 ml of F-5 type acidic film fixing solution, 20 ℃ fixing 15 minutes. Take out the film and rinse it under running water for more than 30 minutes.
(5) Hang up the film, gently absorb the excessive water on the surface of the film with a cotton pad, and let it dry.
(6) After drying with the enlarger film clamps clamp the film, film side down, focusing on the scene, and then in the red light conditions, with the pressure cardboard pressure residence needs to be enlarged like paper, film side up. Exposure (the appropriate exposure time to be decided after the test).
(7) after exposure like paper, immersed in D-72 developer, and then use bamboo tweezers from time to time to clamp like paper flip, until the development of moderate.
(8) after the development of suitable immediately moved to the SB-I type stopping solution, rinse 10 ~ 20 seconds, and then immersed in the fixing solution for 15 minutes, removed in the running water rinse for about 60 minutes.
(9) After rinsing in water, put it on the electric heating varnishing machine to dry; cut the edges and store it in the paper bag with instructions.