Chloroplast subclassification experiments
Chloroplast subclassification experiments
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Operation method
Chloroplast subclassification
Materials and Instruments
Chloroplast Move 1. Pipette a chloroplast suspension containing 1 mg of chlorophyll into a microcentrifuge tube. For more product details, please visit Aladdin Scientific website.
Lysis buffer
Microcentrifuge Tubes Mini Centrifuge
2. Centrifuge in a small centrifuge at 14000 r/min for 30 seconds and discard the supernatant.
3. Add 1 ml of Lysis Buffer, shake, and ice bath for 5 minutes.
Lysis Buffer:
10 mmol/L HEPES-KOH, pH 8.0
4. Centrifuge at 14000 r/min for 2 min in a minicentrifuge and discard the supernatant (matrix-rich fraction).
5. Wash the membrane components with 1x SH at least twice (enriched with vesicle fractions).
Preservation Solution:
20 mmol/L HEPES-KOH, pH 8.0
20 mmol/L sorbitol
125 mmol/L KCl
Contains 30% (v/v) DMSO or 30% ethylene glycol