It is a complementary experiment to experimental protocols A and B of the differential display technique (DD) experiment and the gene expression series analysis experiment.
Modern Neuroscience Research Techniques
Author(s): U. Windhorst & H. Johansson Translated by Z. Q. Zhao Jun Chen
Operation method
Connections for dual-label experiments
Materials and Instruments
Solutions and Buffers LoTE cDNA Labeling
Move
Experimental program A
1. In this experimental protocol, two partially flattened cDNA tags (derived from the same cDNA synthesis reaction) are combined to form a double tag. Add the following substances:
2. Incubate at 16°C overnight.
Experimental protocol B
1. Patch flat labels are combined to form double labels by adding the following substances.
2. Incubate at 16°C overnight.
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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Cite this article
Aladdin Scientific. "Connections for dual-label experiments" Aladdin Knowledge Base, updated Dec 24, 2024. https://www.aladdinsci.com/us_en/faqs/connections-for-dual-label-experiments-en.html
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