Connections for dual-label experiments

Summary

It is a complementary experiment to experimental protocols A and B of the differential display technique (DD) experiment and the gene expression series analysis experiment.

Modern Neuroscience Research Techniques

Author(s): U. Windhorst & H. Johansson Translated by Z. Q. Zhao Jun Chen

Operation method

Connections for dual-label experiments

Materials and Instruments

Solutions and Buffers LoTE cDNA Labeling

Move

Experimental program A

1. In this experimental protocol, two partially flattened cDNA tags (derived from the same cDNA synthesis reaction) are combined to form a double tag. Add the following substances:

2. Incubate at 16°C overnight.

Experimental protocol B

1. Patch flat labels are combined to form double labels by adding the following substances.

2. Incubate at 16°C overnight.

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