Whole blood fluorescence monolabeling can detect platelet CD62p, CD63, CD42b, CD41, CD61 and other indicators. Source: Color Atlas of Practical Flow Cytometry, edited by Shukui Wang and Zhenying Zhou.
Operation method
Detection of platelet surface markers
Principle
Whole blood fluorescent monolabeling for platelet CD62p, CD63, CD42b, CD41, CD61.
Materials and Instruments
Fluorescently Labeled Antibodies Whole Blood Move 1. Blood collection was anticoagulated with citrate or EDTA. For more product details, please visit Aladdin Scientific website.
Citrate EDTA PBS PFA
2. 10 ul fluorescent labeled antibody, add 100 ul PBS, then 5 ul whole blood (sample tube); 10 ul antibody isotype control, add 100 ul PBS, then 5 ul whole blood (control tube); mix gently, and incubate at room temperature for 15 min.
3. Add 2-3 ml of PBS (1% PFA) to terminate the reaction, and test on the machine. Analyze.