Dextrose Agar (Powder)

Directions per Liter:

Suspend 43g of the powder in 1L of distilled water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Optional: To prepare blood agar, aseptically add 5% sterile defibrinated blood to the medium at 45-50°C. Mix well and dispense as desired.

Component	g/liter
Pancreatic Digest of Casein	5
Proteose Peptone No. 3	2
Pancreatic Digest of Gelatin	3
Beef Extract	3
Dextrose	10
Sodium Chloride	5
Agar	15
	Total: 43g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.