EC Medium (Powder)

Directions per Liter:

Dissolve 37grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)

Component	g/liter
Casein Digest Peptone	15
Deoxyribonucleic Acid	2
Papaic Digest of Soybeans	5
Sodium Chloride	5
Agar	15
Toluidine Blue O	0.1
	Total: 37g/Liter

Storage and Stability:

Store powdered media at 4°C Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should stored at 4°C and used within a short period of time.