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Laboratory Protocols & SOPs Library

ELISA Coating Solution Recipe

· Published 27.07.2023 · Updated 09.06.2025

Recipe

1.Dissolve 5.3g of Na2CO3 in 900ml distilled H2O.

2.Dissolve 4.2g of NaHCO3 in the solution from step 1.

3.Dissolve 1g sodium azide in the solution from step 2.

4.pH to 9.6.

5.Adjust volume to 1L with additional distilled H2O.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "ELISA Coating Solution Recipe" Aladdin Knowledge Base, updated 09.06.2025. https://www.aladdinsci.com/eu_de/faqs/elisa-coating-solution-recipe-en.html

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