Experiments on plant stomatal detection by blotting method
Experiments on plant stomatal detection by blotting method
Through the experiment, master the principle and method of observing the stomatal opening and closing condition of plant leaves by blotting method.
Operation method
Experiments on plant stomatal detection by blotting method
Principle
The organic sol coated on the surface of the plant, the colloid air-drying condensed into a thin film, which film is printed with the epidermal tissue of the cells of the boundary traces, thus showing the opening and closing of the stomata, this method in addition to the observation of the stomata, can also be used for the observation of the epidermal tissues on the cells, velvet, and nectar glands, honey discs, thorns, scales and so on.
Materials and Instruments
Plant leaves Move 1. Take 5-10g of kraft glue, put it into 100ml of water, and heat it to 70-80℃ to make it into sol. If it turns into gel after cooling, add appropriate amount of water and reheat to make it into sol. If you need to keep it for a long time, you can add a few drops of preservative such as toluene on the surface of the sol, which can also prevent the evaporation of water from the sol. For more product details, please visit Aladdin Scientific website.
Degreased cotton wool Kraft glue Toluene Paraffin wax
Microscope Eyepiece Micrometer Slides Coverslips Ground glass vials Brushes or small sticks Dissecting needles Pointed tweezers
2. Apply the sol to the leaf surface to form a thin layer, wait for a few minutes, and remove it with tweezers after the sol dries to a thin film. On a slightly moist slide this film will stick firmly and can be stored for a long time. In order to long-term preservation, can be sealed under dry conditions with a coverslip (adhesive film such as hygroscopic water, the imprint immediately deformed or disappeared, such adhesive film can not meet with glycerol, xylene, anhydrous ethanol and other liquids). No liquid is added when sealing the tablets. Gum arabic can be used directly around the coverslip adhesive solid. In order to avoid the xylene in gum arabic leaching into the coverslip, can also be changed to use paraffin wax, etc. to seal the coverslip.
3. cowhide offset film toughness, needle picking tweezers and sealing film are not easy to break. After air-drying the film is uniform and flat, microscopic analysis is very clear (but if the film layer is too thick, sealing the film is more difficult). In addition, the low cost of the adhesive solution used in kraft adhesive blotting method, the preparation is simple, the speed of the film is moderate, and because it is a water-soluble adhesive, so it does not harm the plant tissues, so it can be used on the surface of the same tissue for continuous blotting samples, in order to observe this part of the tissue epidermal phylogeny.