A peptide can be biotinylated yet still read negative in a biotin assay. The most common root cause is steric hindrance or conformational occlusion: the biotin is positioned too close to the peptide backbone or becomes shielded by the peptide’s structure/aggregation, so streptavidin/avidin ...
Cholesterol is a ubiquitous membrane component in biology. In lipid bilayers it intercalates between phospholipids, tightening packing and modulating fluidity, elasticity, and permeability. In simple terms, it can “fill” packing defects created by imperfectly ordered lipids or by embedded ...
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