How do I set up an FMO combined isotype control?

How do I set up an FMO combined isotype control?

It is recommended to do FMO-ISO (FMO-ISO) for indicators with low expression or less distinctive clustering, and only one tube is needed. Take IFN-γ as an example, take the “sample of the group expected to express more IFN-γ” as the sample of FMO-ISO, and add other flow antibodies except IFN-γ and IFN-γ isotype control antibody, this tube of sample is called the FMO combined isotype control tube. The rest of the experimental steps were consistent with the experimental group, such as dead-activation and fixation of ruptured membranes.

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