NZC Broth (Powder)

Directions per Liter:

Dissolve 25.94 grams per liter of sterile distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
NZ Amine	10
Casamino Acidse	10
NaCl	5
MgCl2	0.94
	Total: 25.94g/Liter
	500g makes 19.3 liters

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.