Papanicolaou's Stain

Recipe

1.Mix 11.25ml of a 10% eosin Y solution into 450ml of 100% ethanol.

2.Mix 2.25ml of a 10% aqueous solution of light green SF into the solution from step 1.

3.Mix 25ml of a 1% bismarck brown solution (in ethanol) into the solution from step 2.

4.Mix 10ml of a 10% aqueous solution of phosphotongstic acid into the solution from step 3.

5.Mix 0.3ml of an aqueous solution of saturated lithium carbonate into the solution from step 4.