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Phosphate Buffered Saline (PBS) 10x

Protocols

Phosphate Buffered Saline (PBS) 10x

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

1.Dissolve the following in 800ml distilled H2O.

80g of NaCl
2.0g of KCl
14.4g of Na2HPO4
2.4g of KH2PO4

2.Adjust pH to 7.4.

3.Adjust volume to 1L with additional distilled H2O.

4.Sterilize by autoclaving

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Phosphate Buffered Saline (PBS) 10x" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/phosphate-buffered-saline-pbs-10x-en.html

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