Recombinant Human ATP6V1G3 Protein: Key FAQs

Recombinant Human ATP6V1G3 Protein is a recombinant form of the G3 subunit of vacuolar-type H⁺-ATPase (V-ATPase), produced for use in biochemical and cell biology research applications including enzyme assembly studies, interaction assays, and lysosomal acidification investigations.

Gene Name

ATP6V1G3

Protein Full Name

V-type proton ATPase subunit G 3 (Vacuolar proton pump subunit G 3)

Protein Complex

V1 domain of vacuolar-type H⁺-ATPase (V-ATPase)

Species Origin

Human (Homo sapiens)

Product SKU

rp369799

Supplier Reference

absea#PP-4151

Intended Use

Research use only (RUO)

What Is ATP6V1G3 and Why Is It Significant?

ATP6V1G3 encodes the G3 isoform of the G subunit of the vacuolar-type H⁺-ATPase (V-ATPase) V1 catalytic domain. V-ATPase is a multi-subunit proton pump responsible for acidifying intracellular compartments such as lysosomes, endosomes, and secretory vesicles, as well as the extracellular environment in specialized tissues including the kidney and inner ear.

The G subunit exists as three tissue-specific isoforms (G1, G2, G3) in humans. ATP6V1G3 expression has been reported in specific tissues, and its distinct isoform may contribute to tissue-specific regulation of V-ATPase activity. Dysregulation of V-ATPase subunits is associated with diseases such as renal tubular acidosis, osteoporosis, and neurodegenerative conditions, making ATP6V1G3 a relevant target for mechanistic studies.

Product Specifications and Quality Attributes

Recombinant Human ATP6V1G3 Protein (SKU: rp369799) is produced to research-grade standards. Researchers should consult the product datasheet for confirmed details on the following attributes, as specifications may vary by lot:

• Expression System: Please refer to the product datasheet for the confirmed expression host (e.g., E. coli, baculovirus, or mammalian system).
• Tag Information: The recombinant construct may carry an affinity tag (such as His-tag) to facilitate purification; confirm tag position and identity on the datasheet.
• Purity: Typically assessed by SDS-PAGE; target purity level and verification method are stated on the Certificate of Analysis (CoA).
• Formulation: Supplied in a defined buffer; refer to the datasheet for exact buffer composition, pH, and any additives such as glycerol or BSA.
• Molecular Weight: Calculated molecular weight of ATP6V1G3 can be confirmed on the product datasheet, accounting for any tag sequences.

For lot-specific data including endotoxin levels, bioactivity results, and SDS-PAGE images, request the Certificate of Analysis from Aladdin Scientific customer service.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. General best-practice guidelines for Recombinant Human ATP6V1G3 Protein are outlined below; always defer to the product-specific datasheet for authoritative instructions:

• Short-term storage (up to 1 month): Store at 4 °C if the product will be used promptly after receipt.
• Long-term storage: Store at −20 °C or −80 °C (as specified on the datasheet). Aliquot the protein into single-use volumes before freezing to avoid repeated freeze-thaw cycles, which can cause aggregation and loss of activity.
• Freeze-thaw cycles: Minimize freeze-thaw cycles; a maximum of 2–3 cycles is a general industry recommendation. Adding a carrier protein (e.g., 0.1% BSA) to dilute working stocks may improve stability.
• Reconstitution: If supplied lyophilized, reconstitute in sterile deionized water or the buffer specified on the datasheet. Allow the vial to equilibrate to room temperature before opening, and gently mix without vortexing to prevent denaturation.
• Working concentrations: Determine empirically for each application. Protein concentration should be measured using a validated method (e.g., BCA assay, A280) before use.

Research Applications of Recombinant Human ATP6V1G3 Protein

Recombinant Human ATP6V1G3 Protein is intended for research use only and supports a range of in vitro biochemical and cell biology experiments. Validated applications should be confirmed on the product datasheet. Potential research applications include:

• Protein–Protein Interaction Studies: Use as bait or prey in co-immunoprecipitation (Co-IP) or pull-down assays to map interactions with other V-ATPase subunits or regulatory partners.
• Structural Biology: Suitable as a component for reconstitution of partial or complete V-ATPase V1 complexes for structural characterization by cryo-EM, X-ray crystallography, or SAXS.
• Antibody Validation: Use as a positive control antigen to validate anti-ATP6V1G3 antibodies in western blot, ELISA, or immunoprecipitation.
• Enzyme Activity Assays: As part of reconstituted V-ATPase complexes to assess ATPase activity and the contribution of the G3 isoform to catalytic function.
• Drug Discovery and Screening: V-ATPase is an emerging therapeutic target; recombinant subunits can support inhibitor binding and screening assays.
• Functional Genomics Rescue Experiments: Supplement knockdown or knockout cellular models with exogenous protein to confirm on-target phenotypes.

Ordering, Quality Assurance, and Support

Recombinant Human ATP6V1G3 Protein is available from Aladdin Scientific under SKU rp369799. The following information is provided to assist with procurement and quality assurance decisions:

• Reference Number: This product is sourced under supplier reference absea#PP-4151.
• Certificate of Analysis (CoA): A lot-specific CoA is available upon request. Contact Aladdin Scientific customer service with your lot number to obtain purity data, endotoxin results, and activity data.
• Regulatory Status: This product is for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical use.
• Returns and Replacements: Please consult Aladdin Scientific's returns and refunds policy for eligibility criteria. Products that have been improperly stored or used outside recommended conditions may not be eligible for replacement.
• Technical Support: For application-specific guidance, protocol optimization, or troubleshooting, contact the Aladdin Scientific technical support team via the website or customer service channels.
• Custom Requirements: If larger quantities, custom formulations, or specific activity testing is required, please contact Aladdin Scientific to discuss custom order options.

Frequently asked questions

What is the biological function of ATP6V1G3?

ATP6V1G3 encodes the G3 isoform of the G subunit of vacuolar-type H⁺-ATPase (V-ATPase). The G subunit forms part of the peripheral stalk of the V1 domain and plays a structural role in connecting the V1 catalytic head to the V0 proton-translocating domain. V-ATPase drives proton translocation to acidify intracellular organelles and extracellular compartments, which is essential for endosomal trafficking, lysosomal degradation, and bone resorption.

How should Recombinant Human ATP6V1G3 Protein be stored to maintain stability?

For short-term use, store at 4 °C. For long-term storage, aliquot into single-use volumes and store at −20 °C or −80 °C as directed on the product datasheet. Avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity. Always consult the lot-specific datasheet for authoritative storage conditions.

What applications is Recombinant Human ATP6V1G3 Protein validated for?

Potential applications include protein–protein interaction studies (Co-IP, pull-down), antibody validation (western blot, ELISA), structural biology reconstitution experiments, enzyme activity assays within reconstituted V-ATPase complexes, and use as a reference standard. Validated applications are confirmed on a lot-specific basis and should be checked on the product datasheet or Certificate of Analysis.

How do I obtain a Certificate of Analysis (CoA) for SKU rp369799?

Contact Aladdin Scientific customer service with your product lot number to request a lot-specific Certificate of Analysis. The CoA will contain purity assessment data (typically SDS-PAGE), endotoxin levels, and any available bioactivity data specific to your lot.

Is Recombinant Human ATP6V1G3 Protein suitable for clinical or diagnostic use?

No. Recombinant Human ATP6V1G3 Protein (rp369799) is designated for research use only (RUO). It has not been evaluated for safety, efficacy, or quality standards required for diagnostic, therapeutic, or clinical applications and must not be used for such purposes.

What is the difference between ATP6V1G1, ATP6V1G2, and ATP6V1G3?

ATP6V1G1, G2, and G3 are three tissue-specific isoforms of the G subunit of V-ATPase. While they share structural roles within the V1 peripheral stalk, they differ in expression patterns: G1 is broadly expressed, while G2 and G3 show more tissue-restricted expression. These isoform differences are thought to contribute to tissue-specific regulation of V-ATPase function, though detailed isoform-specific functional data should be confirmed from primary literature.

References

1. Forgac M. Vacuolar ATPases: rotary proton pumps in physiology and pathophysiology. Nature Reviews Molecular Cell Biology. 2007;8(11):917–929. doi:10.1038/nrm2272
2. Muench SP, Trinick J, Harrison MA. Structural divergence of the rotary ATPases. Quarterly Reviews of Biophysics. 2011;44(3):311–356. doi:10.1017/S0033583510000338
3. Kawasaki-Nishi S, Nishi T, Forgac M. Proton translocation driven by ATP hydrolysis in V-ATPases. FEBS Letters. 2003;545(1):76–85. doi:10.1016/S0014-5793(03)00396-X
4. Supek F, Supekova L, Mandiyan S, Pan YC, Nelson H, Nelson N. A novel accessory subunit for vacuolar H+-ATPase from chromaffin granules. Journal of Biological Chemistry. 1994;269(39):24102–24106. PMID:7929066

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.