Recombinant Human ATXN7L1 Protein: Key Facts & FAQs

Recombinant Human ATXN7L1 Protein is a recombinant form of the human Ataxin-7-Like 1 protein, a subunit associated with the SAGA-type chromatin-remodeling and transcriptional co-activator complexes, produced for use in biochemical and cell biology research applications.

Product Name

Recombinant Human ATXN7L1 Protein

SKU

rp374761

Protein Family

Ataxin-7-like (ATXN7L) family, SAGA complex-associated subunit

Species Origin

Human (Homo sapiens)

Supplier Reference

absea#PP-8525

Intended Use

Research use only (RUO); not for diagnostic or therapeutic use

What Is ATXN7L1 and Why Is It Studied?

ATXN7L1 (Ataxin-7-Like 1) is a member of the ataxin-7-like protein family. Ataxin-7 and its related proteins are known to be integral components of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a highly conserved multi-subunit transcriptional co-activator and chromatin-remodeling complex found in eukaryotes. The SAGA complex plays critical roles in histone acetylation, deubiquitination, and the regulation of RNA polymerase II-dependent transcription.

ATXN7L1 is of research interest due to its structural homology with ataxin-7, the protein whose polyglutamine expansion causes spinocerebellar ataxia type 7 (SCA7). Studying ATXN7L1 helps researchers understand the functional redundancy and specialization within the ataxin-7-like subfamily and their collective contributions to gene expression regulation. Recombinant production of ATXN7L1 enables in vitro studies of protein–protein interactions, complex assembly, and enzymatic assays without dependence on endogenous expression systems.

Product Specifications and Quality

The Recombinant Human ATXN7L1 Protein (SKU: rp374761) is manufactured as a recombinant protein derived from the human ATXN7L1 gene. This product is referenced under supplier code absea#PP-8525. As with all Aladdin Scientific recombinant proteins, this reagent is intended exclusively for research use and is not approved for diagnostic, therapeutic, or veterinary applications.

Researchers should consult the product datasheet associated with SKU rp374761 for confirmed specifications including host expression system, tag information, molecular weight, purity grade, formulation buffer, and recommended storage conditions. Specific lot-dependent quality control data such as SDS-PAGE purity profiles and activity assay results are provided with each lot upon shipment.

Recommended Applications

Recombinant Human ATXN7L1 Protein is suitable for a range of biochemical and cell biology research applications. Common uses include:

• In vitro binding and protein–protein interaction assays: Investigating ATXN7L1 interactions with other SAGA complex subunits or binding partners.
• Antibody production and validation: Use as an immunogen or as a positive control antigen in Western blot, ELISA, or dot-blot assays.
• Structural studies: As a substrate for biophysical characterization techniques such as size-exclusion chromatography or analytical ultracentrifugation.
• Functional biochemical assays: Exploring ATXN7L1's role in chromatin remodeling or transcriptional regulation in reconstituted systems.

Please verify suitability for your specific application by referring to the product datasheet or contacting Aladdin Scientific technical support.

Storage, Handling, and Stability

Recombinant proteins require careful handling to maintain activity and integrity. General best-practice guidelines for recombinant proteins of this class include:

• Short-term storage (up to 1 month): Store at 4 °C in the supplied buffer. Avoid repeated freeze–thaw cycles.
• Long-term storage: Aliquot the protein upon receipt and store at −20 °C or −80 °C as indicated on the product datasheet. Single-use aliquots are strongly recommended.
• Handling: Keep on ice during experimental setup. Avoid vortexing; mix gently by pipetting.
• Carrier protein: If the product is supplied in low-concentration form, consider adding a carrier protein (e.g., BSA) per datasheet recommendations to prevent adsorptive losses.

Note: Always refer to the specific lot datasheet supplied with SKU rp374761 for definitive storage instructions, as formulation details may vary by lot.

Ordering and Customer Support

Recombinant Human ATXN7L1 Protein is available from Aladdin Scientific under SKU rp374761. To place an order or request a quotation, visit the Aladdin Scientific website and search by SKU or product name, or contact your regional sales representative.

For technical questions regarding applications, compatibility, or custom formulation requirements, Aladdin Scientific's technical support team is available to assist. Please have the SKU and lot number ready when contacting support to ensure the most accurate guidance.

Bulk quantities, custom concentrations, or alternative formulations may be available upon request. Lead times for specialty configurations may vary; please inquire in advance for time-sensitive projects.

Frequently asked questions

What is the biological function of ATXN7L1?

ATXN7L1 (Ataxin-7-Like 1) is a member of the ataxin-7-like protein family and is associated with the SAGA transcriptional co-activator complex. It is implicated in chromatin remodeling, histone modification, and transcriptional regulation in eukaryotic cells. Its structural homology with ataxin-7 makes it relevant to research on neurodegenerative diseases such as spinocerebellar ataxia type 7 (SCA7).

Which expression system is used to produce rp374761, and what tag does the recombinant protein carry?

Specific details regarding the expression host system and affinity tag for SKU rp374761 are provided on the product datasheet. Please download the datasheet from the Aladdin Scientific product page or contact technical support for confirmed lot-specific information, as these parameters can vary between product versions.

What quality control tests are performed on Recombinant Human ATXN7L1 Protein?

Quality control procedures for recombinant proteins at Aladdin Scientific typically include SDS-PAGE analysis to confirm molecular weight and purity, and where applicable, functional activity assays. Lot-specific QC data are included with each shipment. Please refer to the certificate of analysis (CoA) for SKU rp374761 for confirmed QC results for your lot.

How should I store Recombinant Human ATXN7L1 Protein to maximize stability?

For optimal stability, aliquot the protein upon receipt to avoid repeated freeze–thaw cycles, and store long-term at −20 °C or −80 °C as specified on the product datasheet. For short-term use within one month, storage at 4 °C in the supplied buffer is generally acceptable. Always consult the lot-specific datasheet for definitive storage guidance.

Is Recombinant Human ATXN7L1 Protein suitable for use as a Western blot positive control?

Recombinant Human ATXN7L1 Protein can potentially serve as a positive control antigen in Western blot or ELISA experiments to validate anti-ATXN7L1 antibodies. However, suitability depends on the tag, denaturation conditions, and the specific antibody used. We recommend confirming compatibility with your antibody clone and assay conditions before use in critical experiments.

How do I place an order for rp374761 and what is the typical lead time?

You can order Recombinant Human ATXN7L1 Protein (rp374761) directly through the Aladdin Scientific website by searching the SKU, or by contacting your regional sales representative or customer service team. Lead times vary depending on stock availability and your location; please contact Aladdin Scientific sales for up-to-date availability and estimated delivery timelines.

References

1. Grant PA et al. (1997). Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex. Genes Dev. 11(13):1640-1650.
2. Helmlinger D and Tora L (2017). Sharing the SAGA. Trends Biochem Sci. 42(11):850-861.
3. Aladdin Scientific product page: Recombinant Human ATXN7L1 Protein, SKU rp374761, supplier reference absea#PP-8525.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.