Recombinant Human C2orf76 Protein: FAQs & Technical Guide

Recombinant Human C2orf76 Protein is a research-grade recombinant protein derived from the human C2orf76 gene, offered by Aladdin Scientific to support biochemical, cell biological, and functional studies requiring a well-characterized human protein reagent.

Product Name

Recombinant Human C2orf76 Protein

SKU

rp375834

Species Origin

Human (Homo sapiens)

Protein Target

C2orf76 (Chromosome 2 Open Reading Frame 76)

Product Form

Recombinant protein supplied as lyophilized powder or liquid (verify with datasheet)

Supplier

Aladdin Scientific

Reference Number

absea#PP-9482

What Is C2orf76 and Why Is It Studied?

C2orf76, or Chromosome 2 Open Reading Frame 76, is a protein-coding gene located on human chromosome 2. While its precise biological function is still being elucidated, open reading frame proteins of this class are subjects of growing interest in proteomics, functional genomics, and disease-association studies. Researchers use recombinant forms of such proteins to investigate protein–protein interactions, develop antibody validation tools, and establish functional assay systems.

The availability of a recombinant human C2orf76 protein allows investigators to study its structure and potential roles without relying solely on endogenous expression, which may be low or context-dependent in standard cell lines.

Expression System, Purity, and Formulation

Recombinant proteins can be produced in a variety of expression systems including E. coli, insect cells (baculovirus), mammalian cells (HEK293, CHO), and yeast. The specific expression host used for rp375834 should be confirmed by consulting the official Aladdin Scientific product datasheet, as the expression system affects post-translational modifications, folding, and biological activity.

Purity is typically assessed by SDS-PAGE and may also be evaluated by HPLC. For most research applications, a purity of ≥90% is considered acceptable; however, highly sensitive assays may require ≥95% purity. Endotoxin levels are an additional quality parameter relevant to cell-based assays. Please refer to the product-specific Certificate of Analysis (CoA) for lot-specific purity and endotoxin data.

The protein is generally supplied either as a lyophilized powder for extended stability or as a liquid formulation in an optimized buffer. Confirm the exact formulation details from the product datasheet prior to use.

Storage, Handling, and Reconstitution

Proper storage is critical to maintaining the activity and integrity of recombinant proteins. As a general guideline for lyophilized recombinant proteins:

• Long-term storage: Store at −20 °C or −80 °C as specified on the datasheet.
• Avoid repeated freeze–thaw cycles: Aliquot the reconstituted protein into single-use volumes to prevent activity loss.
• Reconstitution: Reconstitute lyophilized protein in sterile deionized water or the buffer recommended in the datasheet. Allow the vial to equilibrate to room temperature before opening, then add diluent gently and avoid vortexing.
• Working concentration: Centrifuge briefly after reconstitution and determine the appropriate working concentration based on your specific assay.

Always refer to the product-specific datasheet for authoritative storage and reconstitution instructions, as conditions may vary by lot or formulation.

Recommended Applications and Experimental Use

Recombinant human C2orf76 protein is intended for research use only (RUO) and is not approved for diagnostic or therapeutic use. Typical research applications for recombinant proteins of this class may include:

• Antibody production and validation: Use as an immunogen or as a positive control antigen in ELISA and Western blot assays.
• Protein interaction studies: Co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR) to map binding partners.
• Functional assays: In vitro biochemical assays to characterize enzymatic or signaling activity, if applicable.
• Structural studies: Crystallography or cryo-EM sample preparation (suitability depends on protein quality and formulation).

Researchers should perform pilot experiments to optimize protein concentration, buffer conditions, and assay format for their specific experimental needs. Consult the product datasheet for any validated application data provided by Aladdin Scientific.

Ordering, Quality Assurance, and Technical Support

Recombinant Human C2orf76 Protein is available from Aladdin Scientific under SKU rp375834. To place an order, visit the Aladdin Scientific website, search by SKU or product name, and select the desired quantity. For bulk or custom orders, contact the Aladdin Scientific sales team directly.

Each lot of recombinant protein is accompanied by a Certificate of Analysis (CoA) that documents purity, concentration, and quality control test results. Customers are encouraged to request the CoA for their specific lot prior to or at the time of purchase.

For technical questions regarding reconstitution protocols, optimal assay conditions, or cross-reactivity data, please contact Aladdin Scientific's technical support team. Return and refund policies apply in accordance with Aladdin Scientific's standard terms; refer to the company's Returns & Refunds policy for details.

Frequently asked questions

What is Recombinant Human C2orf76 Protein and what is its biological function?

Recombinant Human C2orf76 Protein is a laboratory-produced form of the protein encoded by the human C2orf76 gene located on chromosome 2. The precise biological function of C2orf76 is not yet fully characterized in the scientific literature; it is under active investigation in the context of proteomics and functional genomics. Researchers use the recombinant protein to probe its potential interactions and roles in cellular pathways.

How should I reconstitute and store Recombinant Human C2orf76 Protein (rp375834)?

For lyophilized preparations, reconstitute the protein in sterile deionized water or the buffer specified in the Aladdin Scientific product datasheet, allowing the vial to reach room temperature before opening. After reconstitution, aliquot into single-use volumes and store at −20 °C or −80 °C as directed, avoiding repeated freeze–thaw cycles to preserve activity. Always consult the product-specific datasheet and CoA for authoritative, lot-specific instructions.

What applications is Recombinant Human C2orf76 Protein validated for?

This recombinant protein is intended for research use only and may be suitable for applications such as ELISA, Western blot (as a positive control or standard), antibody production, pull-down assays, and protein interaction studies. Specific validated applications for rp375834 should be confirmed by consulting the official Aladdin Scientific product datasheet, as validated application data is lot- and product-specific.

What expression system is used to produce rp375834, and does this affect activity?

The expression system used for Recombinant Human C2orf76 Protein (rp375834) should be confirmed on the Aladdin Scientific product datasheet. Common systems include E. coli, insect cells, or mammalian cells, each affecting post-translational modifications and folding differently. For functional assays requiring native-like modifications, a eukaryotic expression system is generally preferred.

How do I order Recombinant Human C2orf76 Protein and what documentation is available?

You can order Recombinant Human C2orf76 Protein directly from the Aladdin Scientific website using SKU rp375834. Each lot is supported by a Certificate of Analysis (CoA) documenting purity, concentration, and QC results; you may request the CoA before purchase. For technical or procurement assistance, contact Aladdin Scientific's support team through the website.

References

1. Aladdin Scientific product page: Recombinant Human C2orf76 Protein, SKU rp375834. Aladdin Scientific.
2. UniProt Knowledgebase entry for human C2orf76 protein. UniProt Consortium.
3. Structural Genomics Consortium and related open reading frame protein characterization literature — general reference for uncharacterized human proteins.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.