Recombinant Human C8orf88 Protein: Handling & Usage Protocol

Recombinant Human C8orf88 Protein is a research-grade recombinant protein requiring careful reconstitution, aliquoting, and storage to maintain full biological activity and ensure reproducible experimental outcomes.

Product Name

Recombinant Human C8orf88 Protein

SKU

rp376455

Species

Human (Homo sapiens)

Protein Origin

Recombinant expression system

Recommended Storage

-20°C or -80°C; avoid repeated freeze-thaw cycles

Application

Research use only; suitable for biochemical and cell-based assays

Materials and Equipment Required

Before beginning, ensure the following materials are available:

• Recombinant Human C8orf88 Protein (SKU: rp376455, Aladdin Scientific)
• Sterile, nuclease-free water or the manufacturer-recommended reconstitution buffer
• Low-binding microcentrifuge tubes (0.5 mL or 1.5 mL)
• Calibrated micropipettes and sterile tips
• Refrigerated microcentrifuge
• Ice bucket or cooling rack
• Protein quantification reagents (e.g., BCA or Bradford assay kit)
• Personal protective equipment (gloves, lab coat, eye protection)

All consumables in contact with the protein should be low-protein-binding grade to minimize adsorption losses.

Reconstitution Procedure

Follow these steps carefully to reconstitute Recombinant Human C8orf88 Protein:

1. Equilibrate the vial: Allow the lyophilized protein vial to equilibrate to room temperature (18–25°C) for 10–15 minutes before opening to minimize condensation.
2. Centrifuge briefly: Spin the sealed vial at 3,000–5,000 × g for 30 seconds to pellet any lyophilized material from the walls and cap.
3. Add reconstitution buffer: Gently add the recommended volume of sterile reconstitution buffer (typically sterile PBS pH 7.4, or as specified on the product datasheet) along the side of the vial to avoid foaming.
4. Mix gently: Do not vortex. Gently swirl or pipette up and down to dissolve the protein completely. Allow the vial to stand for 5 minutes if needed.
5. Verify solubility: The solution should be clear or slightly opalescent. Discard if visible particulates or turbidity persist after gentle mixing.
6. Quantify protein concentration: Use a BCA or Bradford assay to confirm the protein concentration before use.

Note: Specific reconstitution volume and buffer composition may vary. Always refer to the individual product datasheet supplied with SKU rp376455 for definitive instructions.

Storage and Aliquoting Guidelines

Proper storage is critical to preserving the activity of Recombinant Human C8orf88 Protein:

• Short-term storage (up to 1 week): Store at 4°C in the reconstituted form if the protein will be used within 7 days. Add a carrier protein such as 0.1% BSA if the working concentration is very low (<10 µg/mL) to prevent adsorption to tube walls.
• Long-term storage: Aliquot the reconstituted protein into single-use volumes using low-binding microcentrifuge tubes, snap-freeze in liquid nitrogen or a dry ice/ethanol bath, and store at -80°C. Storage at -20°C is acceptable but -80°C is preferred for long-term stability.
• Avoid freeze-thaw cycles: Repeated freezing and thawing significantly reduces protein activity and can cause aggregation. Each aliquot should be thawed only once.
• Lyophilized form: Store the unopened lyophilized vial at -20°C, protected from moisture and light, until required.

Record the date of reconstitution on each aliquot tube. Discard aliquots stored beyond the recommended period or those showing signs of aggregation or loss of activity.

Quality Control and Verification

Before using Recombinant Human C8orf88 Protein in critical experiments, perform the following quality checks:

• Protein concentration: Confirm concentration by BCA or Bradford assay and compare with the certificate of analysis (CoA) value.
• Purity assessment: Run an SDS-PAGE gel under reducing conditions to confirm the expected molecular weight band and assess purity. A single major band indicates an acceptable preparation.
• Identity verification: Western blot using a validated anti-C8orf88 antibody can be performed to confirm protein identity.
• Bioactivity (if applicable): If a functional assay is established for C8orf88 in your laboratory, include a positive control to verify that activity is within the expected range.

Certificate of Analysis: Each lot of rp376455 is accompanied by a CoA specifying purity, concentration, and QC test results. Retain the CoA for experimental records and regulatory compliance.

Experimental Applications and Tips

Recombinant Human C8orf88 Protein (rp376455) is intended for research use only and may be applicable in a range of biochemical and cell-based assay formats. General application guidance is provided below:

• In vitro binding assays: Use the reconstituted protein at the concentration determined by preliminary titration experiments. Include appropriate negative controls.
• Cell-based studies: When adding recombinant protein to cell culture media, ensure the protein is filter-sterilized (0.22 µm) prior to addition and that the carrier or storage buffer components are compatible with cell viability.
• Immunoassays: For use as a standard or capture reagent in ELISA formats, validate the coating concentration and detection antibody pair empirically.
• Working dilutions: Prepare fresh working dilutions from frozen aliquots on the day of the experiment. Avoid storing diluted protein for extended periods.

Disclaimer: This protein is for research use only and is not intended for diagnostic, therapeutic, or veterinary use. Always follow institutional biosafety guidelines when handling recombinant proteins.

Frequently asked questions

How should I reconstitute Recombinant Human C8orf88 Protein (rp376455)?

Allow the vial to reach room temperature before opening, briefly centrifuge to pellet the lyophilized material, then gently add the recommended volume of sterile reconstitution buffer (e.g., PBS pH 7.4) along the vial wall. Swirl gently to dissolve completely without vortexing. Refer to the product datasheet for the exact buffer and volume specifications.

What is the best way to store Recombinant Human C8orf88 Protein to maintain its activity?

For long-term storage, aliquot the reconstituted protein into single-use volumes, snap-freeze, and store at -80°C to avoid repeated freeze-thaw cycles, which can degrade protein activity and cause aggregation. For short-term use within one week, storage at 4°C is acceptable. The lyophilized, unopened vial should be kept at -20°C protected from moisture.

What downstream applications is Recombinant Human C8orf88 Protein suitable for?

Recombinant Human C8orf88 Protein is suitable for use in biochemical binding assays, cell-based studies, and immunoassay formats such as ELISA. Researchers should empirically validate the working concentration and assay conditions for each specific application. This product is for research use only and is not intended for diagnostic or therapeutic purposes.

How can I verify the quality of my Recombinant Human C8orf88 Protein preparation?

Confirm protein concentration by BCA or Bradford assay, then assess purity by SDS-PAGE under reducing conditions. Western blot with a validated anti-C8orf88 antibody can confirm protein identity. The certificate of analysis (CoA) provided with each lot of rp376455 contains lot-specific purity and QC data for reference.

How do I prevent protein loss due to adsorption when working at low concentrations?

Use low-protein-binding microcentrifuge tubes and pipette tips throughout all handling steps. When working at very low protein concentrations (below approximately 10 µg/mL), consider supplementing the working solution with a carrier protein such as 0.1% BSA to minimize non-specific adsorption to surfaces.

References

1. Aladdin Scientific. Recombinant Human C8orf88 Protein, SKU rp376455. Product Datasheet.
2. Wingfield PT. Overview of the purification of recombinant proteins. Current Protocols in Protein Science. 2015;80:6.1.1-6.1.35. doi:10.1002/0471140864.ps0601s80
3. Protein storage and stability guidelines. General best practices for recombinant protein handling in research laboratories.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.