Recombinant Human CEP162 Protein: Research Guide & Protocol

Recombinant Human CEP162 Protein is a key research reagent targeting the centrosomal protein CEP162, which plays a critical role in cilia formation and transition zone assembly in human cells.

Product Name

Recombinant Human CEP162 Protein

SKU

rp373962

Target Protein

CEP162 (Centrosomal Protein 162)

Species

Human (Homo sapiens)

Protein Form

Recombinant

Primary Research Application

Ciliogenesis, centrosome biology, transition zone assembly studies

Biological Background of CEP162

CEP162 (Centrosomal Protein 162) is a centrosome-associated protein with an established role in the initiation of ciliogenesis. It localizes to the distal end of the mother centriole and functions as a key regulator coordinating the transition from a centriole to a ciliary organelle. CEP162 recruits transition zone (TZ) components to the growing ciliary axoneme and has been shown to interact with tubulin and several transition zone proteins, thereby bridging centriole maturation and TZ assembly.

Loss of CEP162 function results in defective cilia formation, underscoring its importance in processes ranging from embryonic development to tissue homeostasis. Research into CEP162 is relevant to understanding ciliopathies—a group of disorders caused by defective cilia—including nephronophthisis and Joubert syndrome-related conditions.

Product Specifications & Storage Guidelines

The Recombinant Human CEP162 Protein (SKU: rp373962) is produced using recombinant expression systems to ensure high batch-to-batch consistency. Researchers should consult the accompanying Certificate of Analysis (CoA) for lot-specific data including purity, concentration, and endotoxin levels.

• Expression Host: Refer to CoA for lot-specific information.
• Purity: Refer to CoA; typically verified by SDS-PAGE.
• Formulation: Supplied in a buffered solution; refer to product datasheet for exact buffer composition.
• Storage: Store at −80°C upon receipt for long-term storage. Avoid repeated freeze-thaw cycles to preserve protein integrity. Aliquot upon first thaw.
• Shelf Life: Refer to the product label and CoA for expiration date.

Always centrifuge vials briefly before opening and handle on ice to minimize protein degradation.

Recommended Handling & Reconstitution Protocol

Proper handling of Recombinant Human CEP162 Protein is essential to maintain its activity and structural integrity. Follow the steps below as a general guideline:

1. Thawing: Thaw the protein vial on ice or at 4°C. Do not thaw at room temperature or in a 37°C water bath, as rapid temperature changes can cause aggregation.
2. Centrifugation: Briefly centrifuge the vial (e.g., 5–10 seconds at low speed) to collect any liquid from the cap before opening.
3. Reconstitution (if lyophilized): If supplied in lyophilized form, reconstitute in sterile deionized water or the buffer recommended in the product datasheet. Allow the lyophilized cake to dissolve gently; do not vortex vigorously.
4. Aliquoting: Divide the protein into single-use aliquots to prevent degradation from repeated freeze-thaw cycles.
5. Dilution: Prepare working dilutions fresh in a compatible assay buffer. Pilot experiments to optimize concentration are strongly recommended for each application.

Carrier proteins such as BSA (0.1%) may be added to dilute solutions to stabilize the protein during storage or assay setup, unless incompatible with the downstream application.

Research Applications

Recombinant Human CEP162 Protein is suitable for a range of in vitro and biochemical research applications:

• Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays, co-immunoprecipitation validation, or surface plasmon resonance (SPR) to characterize CEP162 binding partners at the transition zone.
• Biochemical Activity Assays: Investigate CEP162 interactions with tubulin or transition zone components in solution-based assays.
• Antibody Validation: Serve as a positive control antigen for validating anti-CEP162 antibodies in ELISA, Western blot, or dot blot formats.
• Structural Studies: Support biophysical characterization including circular dichroism (CD) and dynamic light scattering (DLS).
• Drug Discovery: Screen for small molecules that disrupt or stabilize CEP162 interactions relevant to ciliopathy research.

Optimization of protein concentration, buffer conditions, and incubation parameters is recommended for each specific application. Please consult the product datasheet or contact Aladdin Scientific technical support for guidance.

Quality, Safety & Regulatory Considerations

Quality Control: Each lot of Recombinant Human CEP162 Protein is subject to quality control testing. Refer to the Certificate of Analysis (CoA) supplied with your order for lot-specific purity and identity data.

Safety: Although recombinant proteins at research grade generally present low biohazard risk, standard laboratory safety practices should be followed. Wear appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Consult the Safety Data Sheet (SDS) for complete hazard information.

Regulatory Status: This product is for research use only (RUO). It is not intended for use in diagnostic, therapeutic, or clinical procedures. Researchers are responsible for compliance with all applicable institutional biosafety protocols and local regulations.

Disposal: Dispose of protein solutions and contaminated materials in accordance with institutional and local regulations for biological waste.

Frequently asked questions

What is the function of CEP162 in ciliogenesis?

CEP162 is a centrosomal protein that localizes to the distal end of the mother centriole and plays a critical role in initiating ciliogenesis. It recruits transition zone proteins to the nascent cilium and bridges centriole maturation with transition zone assembly, making it essential for proper cilia formation.

How should Recombinant Human CEP162 Protein be stored to maintain activity?

The protein should be stored at −80°C upon receipt for long-term preservation. To prevent activity loss, avoid repeated freeze-thaw cycles by dividing the stock into single-use aliquots upon first thaw. Always handle on ice during experiments.

Is Recombinant Human CEP162 Protein suitable for use in Western blot?

Yes, Recombinant Human CEP162 Protein is suitable as a positive control antigen for Western blot to validate anti-CEP162 antibodies. For optimal results, determine the appropriate loading amount empirically and verify the expected molecular weight band on the blot.

Can this protein be used in pull-down or co-immunoprecipitation validation assays?

Yes, Recombinant Human CEP162 Protein is suitable for protein–protein interaction studies including pull-down assays and co-immunoprecipitation validation experiments. It can serve as bait or prey to characterize CEP162 binding partners involved in ciliogenesis and transition zone biology.

What is the regulatory status of this product?

Recombinant Human CEP162 Protein (SKU: rp373962) is sold for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical applications. Researchers must ensure compliance with institutional biosafety guidelines and local regulations when using this product.

References

1. Wang WJ et al. CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. Nature Cell Biology, 2013.
2. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology, 2017.
3. Aladdin Scientific. Recombinant Human CEP162 Protein, SKU rp373962. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.