Recombinant Human CFAP157 Protein: Properties & Applications

Recombinant Human CFAP157 Protein is a cilia- and flagella-associated recombinant protein designed to support research into axonemal structure, ciliary motility, and related ciliopathy mechanisms.

Product Name

Recombinant Human CFAP157 Protein

SKU

rp373941

Protein Family

Cilia- and Flagella-Associated Proteins (CFAPs)

Species Origin

Human (Homo sapiens)

Primary Research Areas

Cilia biology, ciliopathies, reproductive medicine, cell motility

Format

Recombinant protein (details: see product datasheet)

Biological Background of CFAP157

CFAP157 (Cilia- and Flagella-Associated Protein 157) is a member of the CFAP protein family, a diverse group of proteins that localize to cilia and flagella and contribute to the assembly and function of the axoneme — the microtubule-based core structure of these organelles. Motile cilia and flagella are essential for numerous physiological processes, including mucociliary clearance in the respiratory tract, left-right body axis determination during embryonic development, and sperm motility in male reproduction.

Defects in genes encoding CFAPs, including CFAP157, have been associated with primary ciliary dyskinesia (PCD) and other ciliopathies — hereditary disorders characterized by impaired ciliary structure or function. Understanding the molecular roles of CFAP157 is therefore relevant to both basic cell biology and translational research into ciliopathy-related diseases.

Protein Characteristics and Production

Recombinant Human CFAP157 Protein (SKU: rp373941) is produced using recombinant expression systems to yield a protein suitable for in vitro research applications. Recombinant production enables consistent batch-to-batch quality and defined purity levels, which are critical for reproducible experimental outcomes.

Researchers are advised to consult the official Aladdin Scientific product datasheet for rp373941 for confirmed specifications including molecular weight, expression host, tag information, purity grade, formulation buffer, and recommended storage conditions, as these parameters are essential for experimental design.

Research Applications

Recombinant Human CFAP157 Protein is intended for research use and may be applied in a variety of laboratory contexts, including:

• Protein–protein interaction studies: Investigating the binding partners of CFAP157 within the axonemal complex or associated regulatory networks.
• Antibody validation: Serving as a positive control antigen for anti-CFAP157 antibody characterization in Western blot, ELISA, or immunoprecipitation assays.
• Structural and functional studies: Supporting in vitro biochemical assays aimed at elucidating CFAP157's role in ciliary assembly or motility.
• Ciliopathy research: Providing a tool for mechanistic studies of primary ciliary dyskinesia and related conditions.
• Reproductive biology: Examining CFAP157's potential contribution to sperm flagella structure and male fertility.

All uses are strictly for research purposes only and not for diagnostic or therapeutic applications.

Handling, Storage, and Quality Considerations

Proper handling and storage of recombinant proteins are essential to maintain activity and integrity. General best practices for recombinant proteins include:

• Store according to the conditions specified in the product datasheet (typically at −20 °C or −80 °C for long-term storage).
• Avoid repeated freeze-thaw cycles; aliquot upon first use to minimize degradation.
• Centrifuge briefly before opening to collect the contents.
• Keep on ice during experimental procedures to preserve protein integrity.
• Consult the product datasheet for recommended working concentrations and compatible assay buffers.

For specific purity grades, endotoxin levels, and quality control data for rp373941, please refer to the official Aladdin Scientific product page or contact technical support.

Frequently asked questions

What is CFAP157 and why is it important in research?

CFAP157 (Cilia- and Flagella-Associated Protein 157) is a protein that localizes to cilia and flagella and is involved in axonemal assembly and ciliary motility. It is important in research because mutations in CFAP157 and related genes are linked to ciliopathies such as primary ciliary dyskinesia, as well as male infertility caused by flagellar defects.

What applications is Recombinant Human CFAP157 Protein (rp373941) suitable for?

Recombinant Human CFAP157 Protein is suitable for research applications including protein interaction assays, antibody validation (e.g., Western blot, ELISA, immunoprecipitation), in vitro biochemical studies, and mechanistic investigations of ciliopathies and reproductive biology. It is not intended for clinical diagnostic or therapeutic use.

How should Recombinant Human CFAP157 Protein be stored?

Recombinant proteins are generally stored at −20 °C or −80 °C for long-term stability. Repeated freeze-thaw cycles should be avoided; it is recommended to aliquot the protein upon first use. Please refer to the official product datasheet for rp373941 for the exact recommended storage conditions and shelf life.

Is this product validated for use in specific assays?

Specific validated applications and quality control data for rp373941 are provided in the Aladdin Scientific product datasheet. Researchers should consult the datasheet for confirmed assay compatibility, purity, and functional test results before designing experiments.

Can CFAP157 protein be used for studies related to male infertility?

Yes, CFAP157 is expressed in flagellated sperm cells and is relevant to the study of sperm motility and male reproductive disorders. Recombinant Human CFAP157 Protein can serve as a research tool to investigate the role of CFAP157 in sperm flagella structure and function, contributing to our understanding of male infertility mechanisms.

References

1. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology. 2017;18(9):533-547. doi:10.1038/nrm.2017.60
2. Bustamante-Marin XM, Ostrowski LE. Cilia and mucociliary clearance. Cold Spring Harbor Perspectives in Biology. 2017;9(4):a028241. doi:10.1101/cshperspect.a028241
3. Inaba K. Sperm flagella: comparative and phylogenetic perspectives of protein components. Molecular Human Reproduction. 2011;17(8):524-538. doi:10.1093/molehr/gar034

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.