Recombinant Human CFAP221 Protein: FAQs & Technical Guide

Recombinant Human CFAP221 Protein is a research-grade recombinant protein of significant interest in ciliary biology and motility studies, available from Aladdin Scientific (SKU: rp373905) for use in a range of in vitro research applications.

Product Name

Recombinant Human CFAP221 Protein

SKU

rp373905

Protein Family

Cilia and Flagella Associated Protein (CFAP) family

Species

Human (Homo sapiens)

Format

Recombinant protein produced in an expression system; exact formulation details should be confirmed with product datasheet

Intended Use

Research use only (RUO); not for therapeutic or diagnostic use

What Is CFAP221 and Why Is It Relevant to Research?

CFAP221 (Cilia and Flagella Associated Protein 221) is a protein encoded by the CFAP221 gene in humans. It belongs to the broader family of cilia- and flagella-associated proteins, which play structural and regulatory roles in the assembly and function of motile and primary cilia. Cilia are highly conserved organelles critical to diverse biological processes including mucociliary clearance in the airway, cerebrospinal fluid flow, and reproductive cell motility.

Research interest in CFAP221 is driven in part by its association with ciliopathies — a class of genetic disorders arising from defective cilia function. Understanding the molecular role of CFAP221 may contribute to insights into conditions such as primary ciliary dyskinesia (PCD) and related syndromes. Recombinant Human CFAP221 Protein enables researchers to study protein–protein interactions, perform biochemical assays, and develop or validate antibodies in a controlled in vitro setting.

How Should Recombinant Human CFAP221 Protein Be Stored and Handled?

Proper storage and handling of recombinant proteins is essential to preserve biological activity and avoid degradation. While specific formulation details for rp373905 should be confirmed on the product datasheet, the following general best practices apply to recombinant proteins of this class:

• Temperature: Store at −20 °C or −80 °C for long-term preservation. Avoid repeated freeze–thaw cycles, as these can cause protein aggregation and loss of activity.
• Aliquoting: Upon receipt, prepare single-use aliquots before freezing to minimize freeze–thaw cycles.
• Working stocks: Keep on ice during active use. Do not leave at room temperature for extended periods.
• Reconstitution: If supplied as a lyophilized powder, reconstitute with the buffer specified in the datasheet and allow to equilibrate gently; avoid vigorous vortexing.
• Carrier protein: Consider adding BSA (typically 0.1% final concentration) if storing diluted working solutions to prevent adsorption to tube surfaces.

Always consult the product-specific datasheet provided with your order or available from Aladdin Scientific for authoritative storage conditions.

What Applications Is Recombinant Human CFAP221 Protein Suitable For?

Recombinant Human CFAP221 Protein (rp373905) is intended for research use only and is suited to a range of in vitro biochemical and cell biology applications. Typical applications for recombinant proteins in this family include:

• SDS-PAGE and Western blot: As a positive control or molecular weight standard for experiments detecting endogenous CFAP221.
• ELISA development: As a standard or capture antigen in sandwich or indirect ELISA formats for quantifying CFAP221 levels in biological samples.
• Antibody validation: Confirming specificity of anti-CFAP221 antibodies by dot blot or Western blot.
• Protein–protein interaction studies: Pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) experiments to characterize binding partners.
• Biochemical activity assays: Investigating structural or functional interactions within the ciliary axoneme machinery.

Researchers should verify suitability for their specific experimental system by consulting the datasheet and, if necessary, contacting Aladdin Scientific technical support. Application performance may vary depending on assay conditions.

Quality, Purity, and Regulatory Considerations

Aladdin Scientific is committed to supplying research reagents that meet rigorous quality standards. For Recombinant Human CFAP221 Protein (rp373905), the following considerations apply:

• Purity: Recombinant proteins supplied by Aladdin Scientific are typically analyzed by SDS-PAGE to confirm purity. Specific purity values and acceptance criteria are detailed on the product datasheet. Researchers are encouraged to review the certificate of analysis (CoA) supplied with each lot.
• Endotoxin: For cell-based assays, endotoxin levels are a critical quality attribute. Confirm endotoxin specifications with the CoA or contact technical support.
• Lot-to-lot consistency: Aladdin Scientific performs quality control testing on each production lot. Lot-specific data are available upon request.
• Regulatory status: This product is for research use only (RUO) and is not approved or intended for clinical, diagnostic, or therapeutic applications.

If you require additional quality documentation, such as a material safety data sheet (SDS/MSDS) or specific analytical data, please contact Aladdin Scientific customer service.

Ordering, Shipping, and Returns for rp373905

Recombinant Human CFAP221 Protein (SKU: rp373905) is available to order directly through Aladdin Scientific. The following information is relevant to procurement and logistics:

• Ordering: Place orders via the Aladdin Scientific website, by contacting your regional sales representative, or through approved institutional procurement channels. Reference SKU rp373905 when ordering.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Confirm shipping requirements and estimated delivery times with Aladdin Scientific at the time of order.
• Returns and refunds: Returns are subject to Aladdin Scientific's standard returns and refunds policy. Products must be returned in original condition. Contact customer service within the permitted window after receipt if you experience any product quality issues.
• Pricing and promotions: Current pricing is available on the Aladdin Scientific website or from your sales representative. Institutional pricing, volume discounts, and promotional offers may be available — enquire at the time of purchase.
• Lead time: Check current stock availability on the product page or contact customer service for lead time estimates.

Frequently asked questions

What is the biological function of CFAP221?

CFAP221 is a cilia and flagella associated protein implicated in the structural organization and function of motile cilia and flagella. It is thought to play a role in axonemal assembly or stability, and mutations in the CFAP221 gene have been linked to ciliopathies such as primary ciliary dyskinesia. The precise molecular mechanism is an active area of research.

Can Recombinant Human CFAP221 Protein be used as a Western blot positive control?

Yes, recombinant proteins are commonly used as positive controls in Western blot experiments to confirm antibody reactivity and expected molecular weight. Recombinant Human CFAP221 Protein (rp373905) can serve this purpose for anti-CFAP221 antibody validation. Always verify the expected molecular weight against the datasheet, as recombinant proteins may include tags or truncations that affect migration.

How many freeze–thaw cycles can Recombinant Human CFAP221 Protein withstand?

As a general guideline for recombinant proteins, repeated freeze–thaw cycles should be minimized — ideally no more than three cycles — to preserve protein integrity and biological activity. We recommend aliquoting the protein into single-use volumes upon receipt. Specific stability data for rp373905 should be confirmed from the product datasheet or by contacting Aladdin Scientific technical support.

Is Recombinant Human CFAP221 Protein suitable for cell-based assays?

Suitability for cell-based assays depends on endotoxin levels, protein formulation, and the specific assay design. Endotoxin contamination can confound cell-based results. Researchers should review the endotoxin specification on the CoA and confirm compatibility with their cell system. Contact Aladdin Scientific technical support for guidance on your specific application.

What expression system is used to produce rp373905?

The specific expression system used to produce Recombinant Human CFAP221 Protein (rp373905) — such as E. coli, baculovirus, or mammalian cell expression — should be confirmed from the product datasheet, as this information was not fully detailed in the available source material. The choice of expression system can affect post-translational modifications and folding, which may be relevant to your application.

References

1. Aladdin Scientific product page: Recombinant Human CFAP221 Protein, SKU rp373905.
2. General guidance on recombinant protein storage and handling — textbook-level best practice; no specific citation from source material.
3. CFAP221 gene and protein function — cilia and flagella associated protein family; ciliopathy association. General scientific knowledge; specific primary literature citations require independent verification.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.