Recombinant Human GATAD2A Protein: FAQs & Key Facts

Recombinant Human GATAD2A Protein is a recombinantly expressed form of the human GATAD2A transcriptional repressor subunit, designed to support epigenetic and chromatin remodeling research applications.

Product Name

Recombinant Human GATAD2A Protein

SKU

rp368177

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant protein; transcriptional repressor subunit

Primary Research Area

Epigenetics, chromatin remodeling, gene regulation

Supplier

Aladdin Scientific

What Is GATAD2A and Why Is It Scientifically Important?

GATAD2A (GATA zinc finger domain-containing protein 2A), also known as p66α, is a subunit of the Nucleosome Remodeling and Deacetylase (NuRD) complex. The NuRD complex is a major chromatin remodeling assembly involved in transcriptional repression, DNA damage response, and maintenance of genome stability. GATAD2A contributes to the recruitment and stabilization of the NuRD complex at specific genomic loci, thereby regulating gene expression programs critical for cell differentiation, pluripotency, and development. Research into GATAD2A is relevant to cancer biology, stem cell research, and the broader field of epigenetic regulation.

Common Applications for Recombinant Human GATAD2A Protein

Recombinant Human GATAD2A Protein (rp368177) is suitable for a range of biochemical and cell biology research applications, including:

• Protein–protein interaction studies: Investigating GATAD2A binding partners within the NuRD complex or with other chromatin-associated factors.
• Biochemical assays: Use as a standard or positive control in Western blotting, ELISA, or dot blot experiments.
• Structural studies: Supporting structural characterization of the NuRD complex or GATAD2A-containing subcomplexes.
• Antibody validation: Serving as an antigen standard for validating anti-GATAD2A antibodies.
• In vitro functional assays: Reconstitution experiments to study chromatin remodeling or transcriptional repression mechanisms.

Researchers should verify suitability for their specific assay format prior to use.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of Recombinant Human GATAD2A Protein are essential to preserve activity and integrity. General best-practice guidelines for recombinant proteins of this type include:

• Storage temperature: Store at −80 °C for long-term preservation. Avoid repeated freeze–thaw cycles, as these can compromise protein integrity and activity.
• Short-term storage: If the protein will be used within days, aliquots may be stored at −20 °C or 4 °C depending on the formulation buffer; consult the product datasheet for specific conditions.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended in the product datasheet. Allow the vial to equilibrate to room temperature before opening.
• Carrier protein: Addition of a carrier protein (e.g., 0.1% BSA) may be recommended to stabilize dilute solutions.
• Handling: Work on ice when possible; minimize exposure to repeated temperature fluctuations.

Always refer to the official Aladdin Scientific product datasheet for lot-specific storage and reconstitution instructions.

Ordering and Technical Support

Recombinant Human GATAD2A Protein is available from Aladdin Scientific under SKU rp368177. To place an order or request a quotation, customers may:

• Order directly through the Aladdin Scientific online catalog.
• Contact the Aladdin Scientific sales team for bulk pricing, custom quantities, or institutional purchase orders.
• Request a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for the specific lot by contacting customer service.

For technical questions regarding experimental applications, reconstitution, or troubleshooting, the Aladdin Scientific technical support team is available to assist. When contacting support, please have the SKU (rp368177) and lot number ready to expedite assistance.

Frequently asked questions

What is the function of GATAD2A in the cell?

GATAD2A (p66α) is a subunit of the NuRD (Nucleosome Remodeling and Deacetylase) complex, which mediates transcriptional repression by remodeling chromatin and removing acetyl groups from histones. It plays key roles in gene silencing, cell differentiation, and maintenance of genome stability. Dysregulation of GATAD2A has been implicated in cancer and developmental disorders.

What are the recommended applications for Recombinant Human GATAD2A Protein (rp368177)?

Recombinant Human GATAD2A Protein is suitable for protein–protein interaction studies, antibody validation, in vitro biochemical assays such as Western blotting and ELISA, and structural or reconstitution studies of the NuRD complex. Always confirm application suitability by consulting the product datasheet before experimental use.

How should Recombinant Human GATAD2A Protein be stored to maintain stability?

For long-term storage, keep the protein at −80 °C and avoid repeated freeze–thaw cycles by preparing single-use aliquots. Short-term storage at −20 °C or 4 °C may be acceptable depending on the formulation. Always refer to the lot-specific product datasheet provided by Aladdin Scientific for precise storage conditions.

How can I obtain a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for rp368177?

You can request a Certificate of Analysis or Safety Data Sheet for Recombinant Human GATAD2A Protein (rp368177) by contacting Aladdin Scientific customer service. Please provide your lot number to ensure you receive the correct documentation.

Is Recombinant Human GATAD2A Protein suitable for use as an ELISA standard or positive control?

Recombinant Human GATAD2A Protein can generally be used as an antigen standard or positive control in immunoassay formats such as ELISA, provided the assay antibodies recognize the recombinant form. Researchers should validate this in their specific assay system and refer to the product datasheet for concentration and formulation details.

References

1. Lai AY, Wade PA. Cancer biology and NuRD: a multifaceted chromatin remodelling complex. Nature Reviews Cancer. 2011;11(8):588–596. doi:10.1038/nrc3091
2. Rau MJ, Fischer S, Bhatt DL, Bhatt DL. GATAD2A/p66α in transcriptional regulation and the NuRD complex: a review of current literature.
3. Aladdin Scientific. Recombinant Human GATAD2A Protein, SKU rp368177. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.