Recombinant Human GIMAP7 Protein: Production & Usage Protocol

Recombinant Human GIMAP7 Protein is a GTPase IMAP family member 7 recombinant protein intended for use in immunological, cell biology, and biochemical research applications requiring high-purity, functionally active human protein.

Product Name

Recombinant Human GIMAP7 Protein

SKU

rp374209

Protein Family

GTPase IMAP (GIMAP) family

Species Origin

Human (Homo sapiens)

Recommended Storage

-20°C or -80°C; avoid repeated freeze-thaw cycles

Application

Biochemical assays, functional studies, immunological research

Background and Biological Overview

GIMAP7 (GTPase IMAP Family Member 7) is a member of the GIMAP (GTPase of the immunity-associated protein) family, a group of small GTPases predominantly expressed in immune cells, particularly T lymphocytes and natural killer (NK) cells. The GIMAP family plays important roles in lymphocyte survival, differentiation, and homeostasis. GIMAP7 has been implicated in regulating apoptosis and mitochondrial integrity in immune cells, making it a relevant target for studies related to immune regulation and autoimmune diseases.

Recombinant Human GIMAP7 Protein produced by Aladdin Scientific (SKU: rp374209) enables researchers to investigate GIMAP7 function, protein-protein interactions, GTPase activity, and downstream signaling pathways in a controlled in vitro setting.

Reconstitution and Handling Instructions

Materials Required: Sterile deionized water or the buffer recommended in the product-specific datasheet, microcentrifuge tubes, and appropriate pipettes.

1. Equilibration: Allow the lyophilized protein vial to equilibrate to room temperature for 10–15 minutes before opening to minimize condensation and protein degradation.
2. Reconstitution: Reconstitute the lyophilized Recombinant Human GIMAP7 Protein by adding the volume of sterile water or reconstitution buffer specified on the product datasheet. Gently swirl or pipette to mix — do not vortex vigorously, as this may denature the protein.
3. Incubation: Allow the reconstituted solution to stand for 5–10 minutes at room temperature to ensure complete solubilization.
4. Aliquoting: Prepare single-use aliquots to avoid repeated freeze-thaw cycles, which can compromise protein integrity and biological activity.
5. Centrifugation: Briefly centrifuge the vial (1,000–2,000 × g, 30 seconds) before opening to collect any material from the cap or sides.

Note: Always refer to the lot-specific Certificate of Analysis (CoA) for exact reconstitution volumes and buffer composition.

Storage Conditions and Stability

Short-term storage (up to 1 month): Store reconstituted protein at 4°C if it will be used within one month. Addition of a carrier protein such as 0.1% BSA (bovine serum albumin) is recommended to enhance stability during short-term refrigerated storage.

Long-term storage: For storage beyond one month, aliquot and freeze at -20°C or preferably -80°C. Lyophilized protein, prior to reconstitution, should also be stored at -20°C or -80°C, protected from moisture and light.

Avoid: Repeated freeze-thaw cycles, prolonged exposure to room temperature, and storage in frost-free freezers that undergo temperature cycling.

Shelf life: Refer to the expiration date on the product label and the CoA. Under recommended storage conditions, lyophilized recombinant proteins typically remain stable for 12–24 months from date of manufacture.

Experimental Applications and Assay Guidelines

Recombinant Human GIMAP7 Protein is suitable for several research applications:

• GTPase Activity Assays: GIMAP7 can be evaluated for intrinsic GTPase activity using malachite green or ELISA-based phosphate release assays. Ensure appropriate GTP concentrations and buffer conditions (e.g., Mg²⁺ supplementation) as specified in validated assay protocols.
• Protein-Protein Interaction Studies: Use in co-immunoprecipitation (Co-IP), pull-down assays, or surface plasmon resonance (SPR) to characterize GIMAP7 interactions with binding partners such as other GIMAP family members or mitochondrial proteins.
• Western Blot Standard: Use as a positive control or molecular weight standard for GIMAP7 detection in cell lysate experiments.
• ELISA Development: Suitable as a coating or capture antigen for developing or validating GIMAP7-specific ELISA assays.
• Antibody Validation: Use to confirm antibody specificity in dot blot or Western blot formats.

Note: Optimal protein concentrations must be determined empirically for each assay format. Starting concentrations of 0.1–1 µg per reaction are typical for many biochemical assays, but should be validated by the end user.

Quality Control and Safety Considerations

Quality Control: Each lot of Recombinant Human GIMAP7 Protein is subject to quality control testing prior to release. Standard QC measures typically include:

• SDS-PAGE analysis to confirm molecular weight and purity
• Endotoxin testing (LAL assay) to ensure suitability for cell-based and in vivo applications
• Biological activity testing where applicable

Refer to the Certificate of Analysis (CoA) supplied with each lot for lot-specific QC data including purity percentage, endotoxin levels, and activity confirmation.

Safety Considerations: Recombinant Human GIMAP7 Protein is intended for research use only (RUO) and is not approved for diagnostic or therapeutic use in humans or animals. Standard laboratory safety practices should be followed:

• Wear appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection.
• Dispose of protein-containing materials in accordance with institutional biosafety guidelines.
• Consult the Safety Data Sheet (SDS) for detailed hazard information.

Frequently asked questions

What is the recommended reconstitution buffer for Recombinant Human GIMAP7 Protein?

The recommended reconstitution buffer and volume are specified on the lot-specific Certificate of Analysis (CoA) and product datasheet supplied with each order. Typically, sterile deionized water or a phosphate-buffered saline (PBS)-based buffer is used. Always consult the CoA for exact specifications for your lot.

How should I store Recombinant Human GIMAP7 Protein to maintain its activity?

Lyophilized protein should be stored at -20°C or -80°C prior to reconstitution. After reconstitution, prepare single-use aliquots and store at -80°C for long-term use, or at 4°C for up to one month. Avoid repeated freeze-thaw cycles, as these can significantly reduce protein activity and integrity.

Is Recombinant Human GIMAP7 Protein suitable for use in cell-based assays?

Recombinant Human GIMAP7 Protein may be suitable for cell-based assays provided that endotoxin levels meet the requirements for your specific assay. Endotoxin levels are reported on the CoA for each lot. For cell-based applications, confirm that endotoxin levels are below the threshold required for your cell type and assay format, and consult the product datasheet for verified applications.

What is the biological function of GIMAP7?

GIMAP7 is a member of the GTPase IMAP family, which is expressed predominantly in immune cells such as T lymphocytes and NK cells. Members of the GIMAP family are involved in regulating lymphocyte survival, apoptosis, and mitochondrial integrity. GIMAP7 specifically has been studied in the context of immune cell homeostasis, though its precise molecular mechanisms continue to be an active area of research.

Can Recombinant Human GIMAP7 Protein be used as a Western blot positive control?

Yes, Recombinant Human GIMAP7 Protein is suitable for use as a positive control or molecular weight marker in Western blot experiments designed to detect GIMAP7. Load an appropriate amount (typically 10–100 ng per lane) alongside experimental samples to confirm antibody specificity and expected molecular weight. Refer to the CoA for confirmed molecular weight and purity data.

References

1. Voss AK, Goodnow CC. GIMAP GTPases: A protean family with roles in T lymphocyte survival and homeostasis. Biochemical Society Transactions, 2009.
2. Sandal T, et al. GIMAP proteins: their role in lymphocyte development and homeostasis. Frontiers in Immunology, 2015.
3. Aladdin Scientific. Recombinant Human GIMAP7 Protein, SKU rp374209. Product Datasheet and Certificate of Analysis.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.