Recombinant Human GIMD1 Protein: Research Guide & Applications

Recombinant Human GIMD1 Protein is a research-grade recombinant protein designed to support investigations into GIMD1-mediated biological pathways, offering a well-characterized tool for immunological, cell biological, and biochemical studies.

Product Name

Recombinant Human GIMD1 Protein

SKU

rp376164

Protein Origin

Human (Homo sapiens)

Product Type

Recombinant Protein

Primary Application

In vitro biochemical assays, cell-based functional studies, antibody validation

Storage Recommendation

Store at -80°C; avoid repeated freeze-thaw cycles

Biological Background of GIMD1

GIMD1 (GTPase, IMAP Family Member Domain-Containing Protein 1) belongs to the IMAP family of GTPases, a group of proteins implicated in immune cell regulation and survival signaling. Members of this family are known to play roles in lymphocyte development and function, and dysregulation of IMAP family proteins has been associated with immune-related disorders.

GIMD1, expressed in human tissues, is believed to participate in intracellular signaling cascades that influence cell survival and apoptosis. Its specific molecular interactions and downstream effectors remain an area of active investigation, making recombinant protein reagents critical for elucidating its precise biological roles.

Note: Detailed functional characterization data for GIMD1 are still emerging in the literature. Researchers are encouraged to consult the latest publications for updated mechanistic insights.

Product Specifications and Quality

Recombinant Human GIMD1 Protein (SKU: rp376164) is produced using recombinant expression systems to ensure batch-to-batch consistency and high purity suitable for demanding research applications. The following specifications are general guidelines for research-grade recombinant proteins of this class; researchers should refer to the product-specific Certificate of Analysis (CoA) supplied with each lot for confirmed values.

• Expression System: Refer to product datasheet (prokaryotic or eukaryotic expression system; specific host to be confirmed on CoA).
• Purity: Typically ≥90% by SDS-PAGE; exact purity confirmed per lot CoA.
• Formulation: Supplied in a stabilizing buffer; refer to the lot-specific CoA for exact formulation details.
• Endotoxin Level: Tested and reported on CoA; typically <1.0 EU/μg for research-grade preparations.
• Molecular Weight: Refer to product datasheet for predicted and observed molecular weight.

All specifications are subject to lot-specific verification. Aladdin Scientific recommends requesting the CoA prior to use in critical experiments.

Handling, Storage, and Reconstitution Protocol

Proper handling of Recombinant Human GIMD1 Protein is essential to preserve biological activity and ensure reproducible experimental results. Follow the guidelines below:

Upon Receipt

• Inspect the package immediately upon delivery. Verify the product name, SKU (rp376164), and lot number against the CoA.
• If the product is supplied lyophilized, store at -80°C until use. If supplied in liquid form, also store at -80°C and avoid repeated freeze-thaw cycles.

Reconstitution (Lyophilized Format)

• Allow the vial to equilibrate to room temperature for 10–15 minutes before opening to minimize condensation.
• Reconstitute in sterile, deionized water or the buffer specified on the product datasheet to the recommended concentration.
• Mix gently by pipetting; do not vortex.
• Allow the solution to stand for 5 minutes at room temperature to ensure complete dissolution.

Aliquoting and Storage

• Prepare single-use aliquots to avoid repeated freeze-thaw cycles, which can degrade protein activity.
• Store aliquots at -80°C. Short-term storage (up to 1 week) may be performed at 4°C if a stabilizing carrier protein (e.g., 0.1% BSA) is added.
• Do not store in frost-free freezers due to temperature cycling.

Refer to the lot-specific CoA and product datasheet for any deviations from the above general protocol.

Research Applications

Recombinant Human GIMD1 Protein is suitable for a range of in vitro and cell-based research applications. Representative uses include:

• Biochemical Assays: Use as a substrate or binding partner in GTPase activity assays, protein–protein interaction studies, and pull-down experiments to characterize GIMD1 binding partners.
• Antibody Validation: Serve as a positive control antigen for validating anti-GIMD1 antibodies in Western blotting, ELISA, and immunoprecipitation workflows.
• Cell-Based Functional Studies: Exogenous addition of recombinant GIMD1 may be used to study its effects on immune cell survival, proliferation, or apoptosis in relevant cell line models.
• Structural Studies: May be used as a reference material in structural biology experiments aimed at resolving GIMD1 conformation and interaction interfaces.
• Screening Assays: Suitable for use in high-throughput screening platforms to identify small-molecule modulators of GIMD1 activity.

Researchers should optimize experimental conditions, including protein concentration, buffer composition, and incubation parameters, for each specific application.

Troubleshooting Common Issues

The following troubleshooting guidance addresses common challenges encountered when working with Recombinant Human GIMD1 Protein:

Issue	Possible Cause	Recommended Action
No activity detected	Protein degradation due to improper storage or repeated freeze-thaw	Use freshly prepared aliquots; verify storage conditions; check CoA activity data
Aggregation or precipitation	Incorrect reconstitution buffer or excessive concentration	Reconstitute as specified on datasheet; centrifuge at 10,000 × g for 5 min before use; reduce working concentration
High background in binding assays	Non-specific protein binding to assay surfaces	Add blocking agent (e.g., BSA or casein); optimize washing steps
Unexpected molecular weight on SDS-PAGE	Post-translational modifications or tag interference	Compare with CoA reference band; consider reducing vs. non-reducing conditions
Inconsistent results between lots	Lot-to-lot variability	Request lot reservation; perform bridging studies between lots

For persistent issues not resolved by the above steps, contact Aladdin Scientific technical support with your lot number and CoA.

Frequently asked questions

What is GIMD1 and why is it relevant to immunological research?

GIMD1 is a member of the IMAP family of GTPases, proteins known to be involved in immune cell regulation and survival signaling. Research into GIMD1 may provide insights into lymphocyte biology and immune-related pathologies, making recombinant GIMD1 protein a valuable tool for mechanistic studies.

How should Recombinant Human GIMD1 Protein be stored to maintain activity?

The protein should be stored at -80°C immediately upon receipt. Single-use aliquots should be prepared to avoid repeated freeze-thaw cycles, which can reduce biological activity. Short-term storage at 4°C (up to one week) may be acceptable with addition of a carrier protein such as 0.1% BSA; however, always refer to the lot-specific CoA for definitive storage instructions.

Which experimental applications is Recombinant Human GIMD1 Protein validated for?

Recombinant Human GIMD1 Protein (rp376164) is suitable for in vitro biochemical assays, antibody validation (Western blot, ELISA, immunoprecipitation), cell-based functional studies, and use as a reference material in structural or screening studies. Researchers should optimize conditions for each specific application and consult the product datasheet for application-specific guidance.

How can I verify the purity and quality of the recombinant protein I received?

Each lot of Recombinant Human GIMD1 Protein is accompanied by a Certificate of Analysis (CoA) detailing purity by SDS-PAGE, endotoxin levels, and other quality parameters. Researchers can independently verify purity by running an SDS-PAGE gel and comparing results with the CoA reference data. For additional verification, Western blotting with a validated anti-GIMD1 antibody is recommended.

What should I do if the protein forms aggregates after reconstitution?

Aggregation can occur if the reconstitution buffer is incompatible or the protein concentration is too high. Ensure that you are using the buffer specified on the product datasheet, gently mix without vortexing, and centrifuge the solution at 10,000 × g for 5 minutes at 4°C before use to remove any insoluble aggregates. If aggregation persists, contact Aladdin Scientific technical support.

References

1. Voss, A.K. et al. IMAP family GTPases and immune regulation. (General reference – specific publication details to be verified.)
2. Aladdin Scientific. Product Datasheet: Recombinant Human GIMD1 Protein, SKU rp376164. Aladdin Scientific Internal Document.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.