Recombinant Human GLYATL1 Protein: Properties & Applications

Recombinant Human GLYATL1 Protein is a research-grade recombinant enzyme belonging to the glycine N-acyltransferase-like family, produced for use in studies of amino acid conjugation, acyltransferase activity, and related metabolic pathways.

Product Name

Recombinant Human GLYATL1 Protein

SKU

rp372888

Protein Family

Glycine N-acyltransferase-like (GLYAT) family

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant enzyme / acyltransferase

Primary Research Use

Enzyme activity assays, metabolic pathway studies, biochemical characterization

Background and Biological Function

GLYATL1 (Glycine N-Acyltransferase-Like 1) is a member of the GLYAT enzyme family, which catalyzes the conjugation of acyl-CoA thioesters with amino acids such as glycine. This conjugation reaction plays a central role in the detoxification and excretion of aromatic and short-chain fatty acids, as well as in general amino acid metabolism. The GLYAT family enzymes are expressed in the liver and kidney, tissues that are principal sites of xenobiotic metabolism and amino acid conjugation in humans.

GLYATL1 is closely related to the canonical GLYAT enzyme but represents a distinct gene product with its own expression profile and substrate preferences. Research into GLYATL1 contributes to the understanding of acyl-CoA biochemistry, phase II biotransformation reactions, and the metabolic fate of endogenous and exogenous organic acids.

Protein Production and Quality

Recombinant Human GLYATL1 Protein (SKU: rp372888) is produced using recombinant expression systems to ensure high purity and batch-to-batch consistency. Recombinant proteins of this type are typically expressed in heterologous host systems such as E. coli, insect cells, or mammalian cells, and are subsequently purified by affinity chromatography to remove host-cell contaminants.

Quality control measures for recombinant proteins generally include SDS-PAGE for purity assessment, Western blot for identity confirmation, and endotoxin testing to ensure suitability for sensitive biological assays. Researchers should consult the specific product datasheet for confirmed expression system, purity grade, formulation buffer, storage conditions, and validated lot-specific data for rp372888.

Research Applications

Recombinant Human GLYATL1 Protein is suitable for a range of in vitro biochemical research applications, including:

• Enzyme activity assays: Measuring acyltransferase activity using defined acyl-CoA and amino acid substrates to characterize kinetic parameters such as K_m and V_max.
• Substrate specificity studies: Determining the range of acyl-CoA thioesters and amino acid acceptors recognized by GLYATL1 compared to related family members.
• Metabolic pathway research: Investigating the role of GLYATL1 in amino acid conjugation pathways and phase II detoxification reactions.
• Protein interaction studies: Identifying binding partners or regulatory proteins that modulate GLYATL1 function.
• Drug metabolism research: Exploring the contribution of GLYATL1 to the biotransformation of drug candidates and environmental xenobiotics.

This recombinant protein is intended for research use only and is not suitable for therapeutic, diagnostic, or veterinary applications.

Storage, Handling, and Reconstitution

Proper storage and handling of recombinant proteins is critical to maintaining activity and preventing degradation. As a general guideline for recombinant proteins of this class:

• Store lyophilized or liquid protein preparations according to the conditions specified on the product datasheet (typically −20°C or −80°C for long-term storage).
• Avoid repeated freeze-thaw cycles by aliquoting the protein upon first use.
• Reconstitute lyophilized protein in the buffer recommended on the datasheet, ensuring complete dissolution before use.
• For optimal results, centrifuge the vial briefly before opening to collect any material from the cap or walls.

Researchers should always refer to the current product datasheet for rp372888 for the most accurate and up-to-date storage and handling instructions specific to this lot.

Frequently asked questions

What is GLYATL1 and why is it relevant to research?

GLYATL1 (Glycine N-Acyltransferase-Like 1) is an enzyme involved in the conjugation of acyl-CoA thioesters with amino acids, a key step in phase II detoxification and amino acid metabolism. It is of interest in studies of xenobiotic biotransformation, metabolic diseases, and acyl-CoA biochemistry.

What are the recommended applications for Recombinant Human GLYATL1 Protein (rp372888)?

This recombinant protein is recommended for in vitro enzyme activity assays, substrate specificity studies, metabolic pathway investigations, and protein interaction studies. It is intended for research use only and is not suitable for clinical or diagnostic purposes.

How should Recombinant Human GLYATL1 Protein be stored to maintain activity?

General best practices include storing the protein at −20°C or −80°C as specified in the product datasheet, avoiding repeated freeze-thaw cycles by preparing single-use aliquots, and reconstituting lyophilized material in the recommended buffer. Always consult the current datasheet for rp372888 for lot-specific instructions.

Is this product suitable for use in cell-based or in vivo studies?

Recombinant Human GLYATL1 Protein (rp372888) is primarily intended for in vitro biochemical assays. Suitability for cell-based assays depends on formulation, purity, and endotoxin levels confirmed in the product datasheet. It is not approved for in vivo or clinical use.

References

1. Matsuo M, et al. Characterization of human glycine N-acyltransferase-like genes. [General reference; specific details to be verified against primary literature.]
2. Nandi DL, Lucas SV, Webster LT Jr. Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization. J Biol Chem. 1979;254(15):7230-7237. [Cited as background on GLYAT family enzymology; applicability to GLYATL1 should be verified.] PMID:458851

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.