Recombinant Human GLYCTK Protein: Key Facts & FAQs

Recombinant Human GLYCTK Protein is a recombinant form of the human glycerate kinase enzyme, produced for use in biochemical and cell biology research applications requiring a well-characterized metabolic kinase reagent.

Product Name

Recombinant Human GLYCTK Protein

SKU

rp374113

Protein Function

GLYCTK encodes glycerate kinase, an enzyme that catalyzes the phosphorylation of D-glycerate to 2-phospho-D-glycerate in the serine and one-carbon metabolic pathways.

Species Origin

Human (Homo sapiens)

Supplier

Aladdin Scientific

Recommended Use

Biochemical assays, enzyme activity studies, metabolic pathway research, and protein interaction studies involving glycerate kinase.

What Is Recombinant Human GLYCTK Protein?

Recombinant Human GLYCTK Protein is a recombinant version of the human glycerate kinase (GLYCTK) enzyme. The GLYCTK gene encodes glycerate kinase, which belongs to the GLYCK family of enzymes and catalyzes the ATP-dependent phosphorylation of D-glycerate to produce 2-phospho-D-glycerate. This reaction is integral to serine metabolism and the broader one-carbon metabolic network. Dysregulation of glycerate metabolism has been implicated in hyperoxaluria and other metabolic disorders, making GLYCTK a protein of significant research interest.

The recombinant protein format produced by Aladdin Scientific (SKU: rp374113) allows researchers to access a defined, reproducible source of GLYCTK activity for in vitro experimentation without reliance on endogenous cellular sources.

Applications of Recombinant Human GLYCTK Protein

Recombinant Human GLYCTK Protein is suitable for a range of research applications:

• Enzyme Activity Assays: Measuring kinase activity using D-glycerate as a substrate in the presence of ATP to study enzymatic kinetics and inhibitor screening.
• Metabolic Pathway Research: Investigating the role of glycerate kinase in serine biosynthesis and one-carbon metabolism.
• Protein–Protein Interaction Studies: Identifying binding partners and interaction networks of GLYCTK within metabolic complexes.
• Disease Mechanism Studies: Exploring links between GLYCTK dysfunction and conditions such as primary hyperoxaluria or other inborn errors of metabolism.
• Structural Biology: Use as a reference protein for structural or comparative biochemical studies.

Researchers should validate the protein's suitability for each specific assay format in their own laboratory conditions.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human GLYCTK Protein are critical to maintaining its activity and integrity:

• Storage Temperature: Recombinant proteins are generally recommended to be stored at –20°C or –80°C for long-term preservation. Avoid repeated freeze–thaw cycles, which can denature the protein and reduce enzymatic activity.
• Aliquoting: Upon receipt, it is best practice to aliquot the protein into single-use volumes to minimize freeze–thaw events.
• Working Conditions: Keep the protein on ice during experimental setup. Use appropriate buffers compatible with kinase activity assays.
• Shelf Life: Refer to the product datasheet (absea#PQ-4910) for lot-specific expiry and stability data.

Note: Specific storage buffer composition, concentration, and validated stability data should be confirmed from the official product datasheet. Please contact Aladdin Scientific technical support for the most current specifications.

Ordering and Quality Information

Recombinant Human GLYCTK Protein is available from Aladdin Scientific under SKU rp374113. When placing an order, please note the following:

• Product Reference: Internal datasheet reference absea#PQ-4910 may be requested for full technical specifications.
• Quantity and Packaging: Available pack sizes and pricing can be confirmed through the Aladdin Scientific website or by contacting your regional sales representative.
• Quality Assurance: Aladdin Scientific applies quality control procedures to recombinant proteins. Specific QC data such as purity assessment (e.g., SDS-PAGE), endotoxin levels, and activity verification should be obtained from the product datasheet.
• Custom Requirements: For bulk quantities or custom formulations, contact Aladdin Scientific customer support directly.

Researchers are encouraged to review the full product datasheet prior to purchase to confirm suitability for their intended application.

Biological Background: GLYCTK and Glycerate Metabolism

The GLYCTK gene (also referred to as GLYCK or glycerate kinase) is conserved across eukaryotes and encodes an enzyme functioning in the catabolism of serine and glycine. Glycerate kinase phosphorylates D-glycerate using ATP as the phosphate donor, yielding 2-phosphoglycerate, which can then enter glycolysis or gluconeogenesis. This positions GLYCTK at a metabolic junction linking amino acid catabolism to central carbon metabolism.

Loss-of-function variants in the glycerate pathway have been associated with D-glyceric aciduria, a rare metabolic disorder. Research into GLYCTK activity is therefore relevant not only to fundamental metabolic biochemistry but also to the study of rare inherited diseases. Recombinant GLYCTK protein provides an accessible tool for mechanistic and therapeutic research in this area.

Frequently asked questions

What is the biological function of the GLYCTK protein?

GLYCTK encodes glycerate kinase, an enzyme that catalyzes the ATP-dependent phosphorylation of D-glycerate to 2-phospho-D-glycerate. This reaction connects serine and glycine catabolism to central carbon metabolism pathways including glycolysis and gluconeogenesis.

What applications is Recombinant Human GLYCTK Protein suitable for?

Recombinant Human GLYCTK Protein is suitable for enzyme activity assays, metabolic pathway studies, protein–protein interaction analyses, disease mechanism research related to glycerate metabolism, and structural biochemistry investigations. Researchers should validate the protein for each specific assay in their own laboratory conditions.

How should Recombinant Human GLYCTK Protein be stored to maintain activity?

Recombinant proteins including GLYCTK should generally be stored at –20°C or –80°C to maintain stability. It is recommended to aliquot the protein into single-use volumes upon receipt to avoid repeated freeze–thaw cycles, which can degrade protein integrity and reduce enzymatic activity. Always consult the product datasheet for lot-specific storage recommendations.

What is the catalog number for Recombinant Human GLYCTK Protein from Aladdin Scientific?

The catalog number (SKU) for Recombinant Human GLYCTK Protein from Aladdin Scientific is rp374113. The associated internal datasheet reference is absea#PQ-4910. Please use these identifiers when placing orders or requesting technical documentation.

Is Recombinant Human GLYCTK Protein suitable for use in inhibitor screening assays?

Recombinant Human GLYCTK Protein can in principle be used in enzyme inhibitor screening assays that measure glycerate kinase activity. However, suitability for specific assay formats should be confirmed using the product datasheet and validated experimentally in the user's own laboratory. Contact Aladdin Scientific technical support for further guidance on assay setup.

References

1. UniProt Consortium. GLYCTK – Glycerate kinase, human (Q9BXS9). UniProt Knowledgebase.
2. NCBI Gene. GLYCTK glycerate kinase [Homo sapiens]. National Center for Biotechnology Information.
3. Aladdin Scientific. Recombinant Human GLYCTK Protein, SKU rp374113, Datasheet absea#PQ-4910.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.