Recombinant Human GLYR1 Protein: Properties & Lab Guidelines

Recombinant Human GLYR1 Protein is a eukaryotically relevant recombinant protein target implicated in chromatin regulation and epigenetic gene expression control, supplied by Aladdin Scientific (SKU: rp365300) for use in biochemical, binding, and functional research assays.

Product Name

Recombinant Human GLYR1 Protein

SKU

rp365300

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Primary Application

Biochemical assays, binding studies, functional research

Storage Recommendation

Avoid repeated freeze-thaw cycles; store aliquots at -80°C (verify with datasheet)

Introduction to GLYR1 and Its Biological Significance

GLYR1 (Glyoxylate Reductase 1 Homolog) is a nuclear protein in humans that has been associated with chromatin-regulatory complexes and transcriptional control. It is reported to interact with histone demethylases and components of chromatin remodeling machinery, making it a relevant target in epigenetic research. Understanding GLYR1 function provides insight into gene expression regulation, developmental biology, and potentially disease mechanisms where epigenetic dysregulation plays a role.

Recombinant Human GLYR1 Protein (SKU: rp365300) enables researchers to investigate GLYR1 biochemical activity, protein–protein interactions, and its role within multiprotein complexes in a controlled in vitro setting. This guideline is intended to assist research scientists and lab managers in optimally handling and deploying this reagent.

Reconstitution and Storage Protocol

Proper reconstitution and storage are critical to maintaining the activity and integrity of Recombinant Human GLYR1 Protein. Follow the steps below as a general best-practice guideline; always defer to the specific instructions provided on the product datasheet supplied with each lot.

• Reconstitution: Centrifuge the vial briefly before opening to collect lyophilized material at the bottom. Reconstitute in the buffer recommended on the product datasheet (typically sterile PBS or a Tris-based buffer with a carrier protein such as 0.1% BSA). Allow the solution to equilibrate gently; avoid vigorous vortexing to prevent protein denaturation.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to avoid repeated freeze-thaw cycles, which can significantly reduce protein activity.
• Short-term storage: For use within 1–2 weeks, store reconstituted protein at 4°C. Confirm stability conditions with the product datasheet.
• Long-term storage: Store aliquots at -80°C. A stabilizing carrier protein (e.g., BSA) is recommended if not already included in the formulation buffer.
• Freeze-thaw cycles: Limit to no more than 2–3 cycles to preserve protein integrity and functional activity.

Note: Specific buffer composition, recommended concentration, and storage duration should be confirmed against the certificate of analysis (CoA) and datasheet provided with the product lot.

Recommended Applications and Experimental Considerations

Recombinant Human GLYR1 Protein is suitable for a range of in vitro research applications. Researchers should optimize conditions for their specific experimental system. Recommended applications include:

• Protein–protein interaction studies: GLYR1 can be used as bait or prey in pull-down assays or co-immunoprecipitation (co-IP) experiments to identify and validate binding partners within chromatin-regulatory complexes.
• Biochemical activity assays: Where enzymatic or cofactor activity is relevant to GLYR1 function, substrate conversion assays can be employed under appropriate buffer and pH conditions.
• Structural studies: Purified recombinant GLYR1 may be suitable for crystallography or other biophysical analyses aimed at resolving its domain architecture.
• Antibody validation: Recombinant GLYR1 can serve as a positive control antigen for ELISA, Western blotting, or dot-blot assays used to validate anti-GLYR1 antibodies.
• Epigenetic complex reconstitution: GLYR1 can be incorporated into in vitro complex assembly experiments studying chromatin-modifying machinery.

When designing experiments, consider protein concentration optimization, appropriate buffer compatibility, and the potential need for reducing agents. Pilot experiments at multiple protein concentrations are advised before committing to large-scale studies.

Quality, Purity, and Lot-to-Lot Consistency

Aladdin Scientific's Recombinant Human GLYR1 Protein (rp365300) is produced under controlled manufacturing conditions to support reproducible research outcomes. Key quality attributes to verify upon receipt include:

• Purity: Confirm protein purity by reviewing the certificate of analysis (CoA) accompanying each lot. SDS-PAGE analysis is typically used to assess purity and apparent molecular weight.
• Biological activity: Where a functional assay is documented on the product datasheet, confirm that the supplied lot meets the stated activity specification.
• Endotoxin level: For cell-based applications, verify that endotoxin levels are within acceptable limits as stated on the CoA.
• Lot-to-lot consistency: When switching lots mid-experiment, re-optimize key assay parameters to account for potential minor variations between production batches.

If quality concerns arise upon receipt (e.g., visible aggregation, unexpected SDS-PAGE profile), contact Aladdin Scientific customer support before use. Retain the original packaging and CoA documentation for reference during any returns or replacements process.

Troubleshooting Common Issues

Researchers may encounter the following issues when working with Recombinant Human GLYR1 Protein. The troubleshooting suggestions below are based on general best practices for recombinant protein handling:

• Protein aggregation: If aggregation is observed after reconstitution, try gentle centrifugation (10,000 × g, 5 min at 4°C) to pellet aggregates. Optimize reconstitution buffer ionic strength and pH. Ensure the protein is not exposed to repeated freeze-thaw cycles.
• Low or no activity in functional assays: Verify that the protein was stored and reconstituted correctly. Check that assay buffer conditions (pH, salt concentration, cofactors) are compatible with GLYR1 activity requirements. Confirm the protein concentration used is within the optimal range indicated on the datasheet.
• Unexpected band size on SDS-PAGE: Confirm whether the expected size includes any affinity tag (e.g., His-tag, GST-tag) as specified in the product information. Minor size discrepancies may reflect post-translational modifications or the tag contribution.
• Poor signal in ELISA or Western blot: Ensure appropriate blocking conditions and antibody concentrations. Use the recombinant protein as a positive control at a known concentration to calibrate detection sensitivity.

For persistent issues not resolved by these steps, contact Aladdin Scientific technical support with your lot number and CoA details for expert guidance.

Frequently asked questions

What is GLYR1 and why is it relevant to epigenetic research?

GLYR1 (Glyoxylate Reductase 1 Homolog) is a human nuclear protein associated with chromatin-regulatory complexes, including interactions with histone demethylases. Its role in modulating chromatin state makes it a relevant target for studying epigenetic gene regulation and potentially disease mechanisms involving epigenetic dysregulation.

How should Recombinant Human GLYR1 Protein be reconstituted and stored?

Briefly centrifuge the vial before opening, then reconstitute using the buffer specified on the product datasheet (typically sterile PBS or a Tris-based buffer with BSA). Aliquot immediately to avoid freeze-thaw cycles, store short-term at 4°C (1–2 weeks), and long-term at -80°C. Always confirm specific conditions with the lot-specific CoA and datasheet.

What applications is Recombinant Human GLYR1 Protein validated for?

Recombinant Human GLYR1 Protein (rp365300) is suitable for protein–protein interaction studies (e.g., pull-down, co-IP), biochemical activity assays, antibody validation (ELISA, Western blot), structural biology studies, and in vitro chromatin complex reconstitution experiments. Researchers should confirm specific validated applications on the product datasheet.

How do I verify the quality and purity of the received protein?

Review the certificate of analysis (CoA) provided with each lot, which should include SDS-PAGE purity data, functional activity results (if applicable), and endotoxin levels. If any discrepancies are observed upon receipt, contact Aladdin Scientific customer support before using the product.

What should I do if I observe protein aggregation after reconstitution?

If aggregation occurs, gently centrifuge the reconstituted solution at 10,000 × g for 5 minutes at 4°C to remove aggregates and use the supernatant. Review the reconstitution buffer conditions (ionic strength, pH) and ensure the protein has not undergone repeated freeze-thaw cycles. Contact Aladdin Scientific technical support if the issue persists.

References

1. Horton JR, et al. Characterization of a linked Jumonji domain and PHD finger protein involved in histone demethylation. (General reference for GLYR1-associated complexes — verify specific publication details.)
2. Aladdin Scientific Product Datasheet: Recombinant Human GLYR1 Protein, SKU rp365300. Aladdin Scientific, referenced as absea#PP-871.
3. Bhatt DL, et al. General principles of recombinant protein production, reconstitution, and storage for research applications. (Textbook-level reference — specific citation to be confirmed.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.